pH值驱动盐酸小檗碱自组装纳米粒的制备、表征及透皮性能评价OA

Objective To prepare berberine hydrochloride self-assembled nanoparticles(BBH-SAN)and realize stable self-assembly of BBH without carrier materials,and to systematically evaluate its physicochemical properties and in vitro transdermal performance.Methods BBH-SAN were prepared using a heat-assisted pH-driven method,with average particle size and polydispersity index(PDI)as evaluation indicators for process optimization.The nanoparticles were characterized by scanning electron microscopy(SEM),Fourier-transform infrared spectroscopy(FT-IR),thermogravimetric analysis(TG),and X-ray diffraction(XRD).Molecular dynamics simulations were employed to elucidate the self-assembly mechanism of BBH-SAN,and in vitro transdermal experiments were conducted to investigate the drug's transdermal performance.Results The prepared BBH-SAN exhibited lamellar sheet structures under SEM,with an average particle size of(385.67±13.50)nm,a PDI of 0.06±0.04,the ζ potential was determined to be(-34.03±1.01)mV,and an encapsulation efficiency of(87.57±0.05)％.XRD results indicated peak reconstruction and reduced crystallinity,while FT-IR revealed broadening and attenuation of characteristic peaks,suggesting a transition of BBH from a regular crystalline state to a low-crystalline aggregated form.Molecular dynamics simulations revealed that the formation of BBH-SAN is a self-assembly process dominated by van der Waals interactions,regulated by electrostatic interactions,and stabilized synergistically by hydrogen-bond networks.In the in vitro transdermal experiments,the cumulative permeation amount of BBH-SAN within 24 h was(61.25±1.78)μg/cm2,and the skin retention(Qs),dermal retention(Qd),and stratum comeum retention(Qsc)of BBH-SAN were all significantly higher than those of BBH(P＜0.001).Conclusion This study achieved the stable self-assembly of berberine hydrochloride without the use of any carrier through a heat-assisted pH-driven method.The resulting nanoparticles exhibit uniform particle size,good dispersibility,excellent thermal stability,and significantly improved transdermal performance,providing a new strategy for the self-assembly delivery of alkaloid drugs.