宽叶匙羹藤中C21甾体类成分及其促葡萄糖摄取活性研究OA
C21 steroidal components of Gymnema latifolium and their glucose uptake promoting activity
目的 研究宽叶匙羹藤Gymnema latifolium中的C21甾体类成分及其促葡萄糖摄取活性.方法 采用硅胶、ODS、Sephadex-LH 20凝胶及半制备高效液相等技术进行分离纯化,通过波谱数据分析鉴定化合物的结构;采用HepG2细胞葡萄糖摄取模型评价化合物的促葡萄糖摄取活性.结果 从宽叶匙羹藤中分离得到18个C21甾体类化合物,分别鉴定为20-O-benzoyl-12-O-cinnamoyl-3 β,5α,8β,12β,14β,17 β,20-heptahydroxy-(20S)-pregn-6-ene-3-O-β-D-allopyranosyl(1 →4)-β-D-oleandropyra-nosyl(1 →4)-β-D-cymaropyranosyl(1→4)-β-D-digitoxopyranoside(1)、20-O-benzoyl-12-O-cinnamoyl-3β,5α,8β,12β,14β,17β,20-heptahydroxy-(20S)-pregn-6-ene(2)、gymnepregoside H(3)、prosapogenin(4)、sylvepregoside E(5)、gymnepregoside G(6)、penupogenin(7)、gymnepregoside I(8)、cissasteroid A(9)、cynsaccatol L(10)、gymnemarsgenin(11)、gnetumoside A(12)、cynanchin F(13)、gagamine(14)、12-O-benzoylsarcostin(15)、cissasteroid B(16)、12,20-O-dibenzoylsarcostin(17)、hemoside A(18).其中化合物1为新化合物,命名为宽叶匙羹藤苷E;其余均为首次从该植物中分离得到.在无明显细胞毒性条件下,化合物1～18给药组葡萄糖摄取量为对照组的0.70～1.73倍,其中化合物4、8～10、12～14、16和17可显著促进HepG2细胞葡萄糖摄取,摄取量为对照组的1.38～1.73倍.结论 宽叶匙羹藤富含C21甾体类成分,部分化合物显示出良好的潜在抗糖尿病活性,值得进一步深入研究.
Objective To investigate the C21 steroids from Gymnema latifolium and their glucose uptake-promoting activity.Methods The compounds were isolated and purified by various chromatographic techniques including silica gel,ODS,Sephadex LH-20 and semi-preparative HPLC,and their structures were elucidated on the basis of spectroscopic data.The glucose uptake-promoting activity of the isolates was evaluated using a HepG2 cellular glucose uptake model.Results A total of 18 C21 steroids were isolated and identified as gymlatifoside E(1),20-O-benzoyl-12-O-cinnamoyl-3β,5α,8β,12β,14β,17β,20-heptahydroxy-(20S)-pregn-6-ene(2),gymnepregoside H(3),prosapogenin(4),sylvepregoside E(5),gymnepregoside G(6),penupogenin(7),gymnepregoside I(8),cissasteroid A(9),cynsaccatol L(10),gymnemarsgenin(11),gnetumoside A(12),cynanchin F(13),gagamine(14),12-O-benzoylsarcostin(15),cissasteroid B(16),12,20-O-dibenzoylsarcostin(17),hemoside A(18).Among them,compound 1 is a new C21 steroid,and compounds 2-18 were isolated from G.latifolium for the first time.At non-cytotoxic concentrations,treatment with compounds 1-18 resulted in glucose uptake levels ranging from 0.70-to 1.73-fold of the control group in HepG2 cells,among which compounds 4,8-10,12-14,16,and 17 significantly promoted glucose uptake,reaching 1.38-to 1.73-fold of the control group.Conclusion G.latifolium is rich in C21 steroids,and some of these compounds exhibit promising potential anti-diabetic activity,warranting further investigation.
卢羽玲;刘美余;陈连;赵秋华;肖昔文;张进燕;卢汝梅
广西中医药大学药学院,广西南宁 530200广西中医药大学药学院,广西南宁 530200广西中医药大学药学院,广西南宁 530200广西中医药大学药学院,广西南宁 530200广西中医药大学药学院,广西南宁 530200广西中医药大学药学院,广西南宁 530200||特色中药民族药广西高校工程研究中心,广西南宁 530200广西中医药大学药学院,广西南宁 530200||特色中药民族药广西高校工程研究中心,广西南宁 530200
医药卫生
宽叶匙羹藤C21甾体宽叶匙羹藤苷E抗糖尿病活性糖摄取
Gymnema latifolium Wall.ex WightC21 steroidal glycosidesgymlatifoside Eanti-diabetic activityglucose uptake
《中草药》 2026 (19)
7519-7531,13
国家自然科学基金项目(82360823)广西自然科学基金项目(2025GXNSFBA069359)广西中医药大学博士启动基金(2023BS012)
评论