反柄紫芝中1个具有抗动脉粥样硬化潜力的新颖杂萜OA

Objective To discover anti-atherosclerotic compounds from Ganoderma cochlear.Methods Silica gel column chromatography,reversed-phase C18 column chromatography,and semi-preparative HPLC were used for isolation and purification,and the structures of the isolates were elucidated by various spectroscopic techniques(1D and 2D NMR).The in vitro anti-atherosclerotic activity of compound was evaluated using an ox-LDL-induced macrophage lipid deposition model,and the expression of phenotype-related protein was detected by Western blotting.Results A previously undescribed meroterpenoid,designated as cochlemeroid A(1),was isolated and identified from G.cochlear.BODIPY and Oil Red O staining showed that compound 1 significantly inhibited ox-LDL-induced macrophage lipid deposition in a dose-dependent manner,accompanied by reduced TC and TG levels.Furthermore,Western blotting results demonstrated that compound 1 downregulated the expression of cluster of differentiation 36(CD36)and class A scavenger receptor(SR-A),while upregulating the expression of the ATP-binding cassette transporter A1(ABCA1).Conclusion In this study,a new meroterpenoid was successfully isolated from G.cochlear.The bioactivity results indicated that compound 1 possessed significant antiatherosclerotic potential.