基于GEO数据库筛选系统性红斑狼疮关键基因及其致病机制研究OA

Objective To screen key genes in the occurrence and development of systemic lupus erythematosus,explore its pathogenic and regulatory mechanisms,and evaluate the clinical application value.Methods Based on the Gene Expression Omnibus(GEO),the RNA-Seq dataset GSE112087 of systemic lupus erythematosus whole blood samples was downloaded,and the genes upregulated in SLE whole blood samples were screened by the limma package of R software;GO and KEGG functional enrichment analysis was performed by using the org.Hs.eg.db and clusterProfiler packages of R software;Protein interaction network was constructed based on the String database and Cytoscape software to locate key genes in its subnetwork;stepwise regression was performed by SPSSPRO to screen out the key genes in the disease process.Results A total of 112 genes were up-regulated in the whole blood samples of patients with systemic lupus erythematosus.The above genes were mainly enriched with immune response,type Ⅰinterferon signaling pathway,tumor necrosis factor signaling pathway,and RLR signaling pathway.The core genes of protein interaction network were located in the protein interaction network:IFI44L,IFI44,IFIT3,ISG15,RSAD2,IFIH1,MX1,OAS1 and OAS2.Two genes were obtained by stepwise regression of SPSS:IFIT3 and ISG15.Conclusion IFIT3 and ISG15 are key genes in the progression of systemic lupus erythematosus,and are expected to become new biomarkers and molecular therapeutic targets in the diagnosis and treatment of the disease.