金粟兰属药用植物叶绿体基因组结构及变异分析OA

To understand the chloroplast genome structure and variation of medicinal plants in Chloranthus,the chloroplast genomes of 5 medicinal plants in Chloranthus were obtained on NCBI,and the characteristics,re-peat sequences,IR region contraction and expansion and variation sites of chloroplast genomes were studied by bioinformatics methods,and the phylogenetic tree was reconstructed.The findings revealed that the chloroplast genomes of 5 species have similar gene content,gene order and GC content,ranging from 157 063 to 158 758 bp,and the total number of genes is 132–136.Mononucleotide,dinucleotide,trinucleotide,tetranucleotide and com-pound repeat sequences were detected in five plants of Chloranthus.Pentanucleotide was only detected in C.an-gustifolius,C.henryi and C.serratus,and hexanucleotide was only detected in C.angustifolius.The boundary ex-pansion and contraction genes of 5 species are quite different.The coding regions of matK,psbK,atpI,petN,rps4,ycf4,petD and other genes,and the non-coding regions of trnQ-UUG,trnS-GCU,trnC-GCA,trnT-GGU,trnL-UAA and other intergenic regions vary greatly.The phylogenetic analysis showed that the genetic relationship between C.fortunei and C.serratus was close,and the genetic relationship between C.angustifolius and the other four species was far.