微波处理对老化侧柏种子活力和生理特性的影响OA
Effects of Microwave Treatment on Vigor and Physiological Characteristics of Aged Platycladus orientalis Seeds
为解决侧柏种子老化、活力不强等问题,以侧柏种子为材料,采用高温高湿法对种子进行人工加速老化处理,测定不同处理侧柏种子的发芽力,并采用 300、500、600 W(分别记为 W3、W5、W6)3 种功率和 10、20、40、60 s(分别记为 T1、T2、T4、T6)4 种时间组合对老化 4 d 的侧柏种子进行微波处理,测定种子发芽率、相对电导率、超氧化物歧化酶(SOD)活性、过氧化物酶(POD)活性及丙二醛(MDA)、可溶性糖(SS)、可溶性蛋白(SP)、三磷酸腺苷(ATP)含量等指标,并运用隶属函数法对不同微波处理组合进行综合评价.结果表明:侧柏种子老化处理后,其发芽率、发芽势、发芽指数、活力指数显著下降,老化 4 d 种子发芽率从 86.67%降至69.67%.随着微波功率和时间的增加,老化侧柏种子的发芽率、抗氧化酶、贮藏营养物质和 ATP 含量呈先增加后降低趋势,相对电导率和 MDA 含量呈先降低后增加趋势.其中 W3T4 处理发芽率、抗氧化酶、贮藏营养物质与 ATP 含量显著高于 CK,相对电导率与 MDA 含量显著低于 CK.高功率 600 W 长时间(W6T6)微波处理对种子萌发有抑制作用.因此,适宜的微波处理对老化侧柏种子萌发有促进作用,其中 300 W、40 s 处理效果最好.
To solve the problems of aging and low vigor of Platycladus orientalis seeds,this study used P.orientalis seeds subjected to artificial accelerated aging via high-temperature and high-humidity methods.Germin-ation indices of P.orientalis seeds under different aging durati-ons were measured.Aged seeds(4 days)were then treated with microwave at three power levels(300 W,500 W,600 W;denoted as W3,W5,W6)and 4 exposure times(10 s,20 s,40 s,60 s;denoted as T1,T2,T4,T6).The following parameters were evaluated:germination percentage,relative electrical conductivity,malondialdehyde(MDA)content,soluble sugar(SS)content,soluble protein(SP)content,superoxide dismutase(SOD)activity,peroxidase(POD)activity,and adenosine triphos-phate(ATP)content.A comprehensive evaluation of different microwave treatments was conducted using the membership function method.The resultsshowed that after aging treatment,the germination percentage,germina-tion potential,germination index,and vigor index of P.orientalis seeds significantly decreased.The germination percentage of seeds aged for 4 days declined from 86.67%to 69.67%.As microwave power and exposure dura-tion increased,the germination percentage,antioxidant enzyme activities,reserve nutrient content,and ATP levels of aged P.orientalis seeds exhibited an initial increase followed by a subsequent decrease.Conversely,relative electrical conductivity and malondialdehyde(MDA)content demonstrated an initial decline followed by a rise.Among all treatments,the 300 W for 40 s(W3T4)protocol significantly enhanced germination percentage,antiox-idant e-nzyme activities,reserve nutrients,and ATP content compared to the control,while simultaneously redu-cing relative electrical conductivity and MDA levels.Prolonged exposure to high-power(600 W)microwave treatment(W6T6)exerted inhibitory effects on seed germination.Consequently,the appropriate microwave treat-ment promotes the germination of aged P.orientalis seeds,with the 300 W 40 s(W3T4)treatment demonstrating the most effective results.
日央姑力·塞买提;高捍东;叶丹;姚芳;田雯;王玉虓
南京林业大学林草学院、水土保持学院,江苏 南京 210037||国家林业和草原局南方林木种子检验中心,江苏 南京 210037||南京林业大学南方现代林业协同创新中心,江苏 南京 210037南京林业大学林草学院、水土保持学院,江苏 南京 210037||国家林业和草原局南方林木种子检验中心,江苏 南京 210037||南京林业大学南方现代林业协同创新中心,江苏 南京 210037南京林业大学林草学院、水土保持学院,江苏 南京 210037||国家林业和草原局南方林木种子检验中心,江苏 南京 210037||南京林业大学南方现代林业协同创新中心,江苏 南京 210037南京林业大学林草学院、水土保持学院,江苏 南京 210037||国家林业和草原局南方林木种子检验中心,江苏 南京 210037||南京林业大学南方现代林业协同创新中心,江苏 南京 210037南京林业大学林草学院、水土保持学院,江苏 南京 210037||国家林业和草原局南方林木种子检验中心,江苏 南京 210037||南京林业大学南方现代林业协同创新中心,江苏 南京 210037南京林业大学林草学院、水土保持学院,江苏 南京 210037||国家林业和草原局南方林木种子检验中心,江苏 南京 210037||南京林业大学南方现代林业协同创新中心,江苏 南京 210037
农业科技
侧柏微波处理种子活力萌发特性生理特性种子老化
Platycladus orientalismicrowave treatmentseed vigorgermination characteristicphysiologic-al charactcristicseed aging
《西南林业大学学报》 2026 (9)
21-28,8
国家林业和草原局科技成果国家级成果推广项目(2020133138)资助.
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