AQP3、AQP7、AQP9的生物学功能及其对配子冷冻保存作用的研究进展OA
Research Progress on the Biological Functions of AQP3,AQP7,AQP9 and Their Role in Gamete Cryopreservation
水通道蛋白(aquaporins,AQPs)作为具有高效选择性的水通道,在缓解冷冻诱导的细胞损伤方面发挥着至关重要的作用.其中,AQP3、AQP7与AQP9是具有特殊底物选择性的多功能通道蛋白,除转运水外,还能高效转运甘油、乙二醇和过氧化氢等小分子,从而参与调控渗透平衡、氧化应激与代谢稳态.配子冷冻是遗传资源长期高效保存的关键技术,然而氧化应激与能量代谢紊乱是冷冻损伤的核心机制,而AQP3、AQP7、AQP9通过转运小分子物质参与这些过程的调控.因此,本文综述了AQP3、AQP7与AQP9在氧化应激及代谢稳态中的调控机制,及其在配子冷冻保存中的研究进展,并介绍了目前靶向调控策略,为开发高效、低毒的配子冷冻保存方案提供理论依据.
Aquaporins(AQPs),as highly selective water channels,play a crucial role in alleviating cell damage induced by freezing.Among them,AQP3,AQP7 and AQP9 are multifunctional channel proteins with special substrate selectivity.Besides transporting water,they can also efficiently transport small molecules such as glycerol,ethylene glycol and hydrogen peroxide,thereby participating in regulating osmotic balance,oxidative stress and metabolic homeostasis.Gamete cryopreservation is a key technology for the long-term and efficient preservation of genetic resources.However,oxidative stress and energy metabolism disorders are the core mechanisms of cryopreservation damage,and AQP3,AQP7,AQP9 directly participate in the regulation of these processes by transporting small molecule substances.Therefore,this review summarizes the regulatory mechanisms of AQP3,AQP7,AQP9 in oxidative stress and metabolic homeostasis,along with their research progress in gamete cryopreservation.It also introduces current targeted regulation strategies,aiming to provide a theoretical basis for the development of efficient and low-toxicity gamete cryopreservation protocols.
齐雅天;房晓欢;李俊杰
河北农业大学动物科技学院,保定 071000河北农业大学动物科技学院,保定 071000||山东畜牧兽医职业学院动物科技学院,潍坊 261061河北农业大学动物科技学院,保定 071000||河北省牛羊胚胎技术创新中心,保定 071000
农业科技
水通道蛋白配子冷冻保存氧化应激能量代谢
aquaporinsgamete cryopreservationoxidative stressenergy metabolism
《畜牧兽医学报》 2026 (9)
4720-4730,11
国家重点研发计划项目(2021YFD1200401)
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