基于电生理信息指标的黄精种质资源差异研究OA

Objective To compare the electrophysiological characteristics of different Polygonatum sibiricum Red.germplasm resources,thereby providing support for their comprehensive utilization and development.Methods Electrophysiological parameters of 18 Polygonatum sibiricum Red.accessions from different regions were measured using an LCR meter.Nine electrophysiological indi‑ces,including leaf intracellular water holding capacity(IWHC),intracellular water use efficiency(IWUE),and intracellular water holding time(IWHT),were calculated based on the Nernst equation.The divergence among these germplasm resources was investi‑gated by combining them with agronomic traits.Results Significant divergence was observed in the traits of the Polygonatum sibiricum Red.germplasm resources.Comprehensive analysis of the electrophysiological indices from the tested accessions was conducted using principal component analysis(PCA)and cluster analysis.Accession No.13 exhibited significantly higher nutrient active transport capacity(NAC)and metabolic activity(MA)compared to the others,while its IWUE,IWHT,and unit nutrient flux(UNF)were lower.This performance profile is consistent with the local climatic environment.Furthermore,different types of Polygonatum sibiricum Red.exhibited similarities at the electrophysiological level.Conclusion Measurement of plant electrophysiological information can be used to calculate water and nutrient metabolism status,thereby enabling the analysis of germplasm divergence in Polygonatum sibiricum Red..This approach provides a basis for the breeding of superior varieties and the improvement of cultivation techniques.