基于"相资为制"的女贞子制生地黄炮制增效作用研究OA

Objective To investigate the processing-induced efficacy enhancement of Radix Rehmanniae prepared by Fructus Ligustri Lucidi and preliminarily explore its underlying mechanism.Methods The processed product was prepared and subjected to quality con‑trol.Indicators including hot zone residence ratio,water intake,anal temperature,body weight,organ indices,and serum levels of tri‑iodothyronine(T3),thyroxine(T4),corticosterone(CORT),cyclic adenosine monophosphate(cAMP),and cyclic guanosine mono‑phosphate(cGMP)were measured to evaluate the yin-nourishing and heat-clearing efficacy.Antidepressant effects were assessed via sucrose preference test(SPT),tail suspension test(TST),forced swimming test(FST),and open field test(OFT),combined with hematoxylin-eosin(HE)staining of hippocampal tissues.Western blot(WB)was performed to detect the protein expression of glucocor‑ticoid receptor(GR)and brain-derived neurotrophic factor(BDNF)in the hippocampus to analyze the efficacy-enhancing mechanism.Results Compared with the normal group,the model group showed significantly decreased hot zone residence ratio,body weight,organ indices,sucrose preference rate,open-field locomotor distance,and hippocampal GR and BDNF protein expression(P<0.01),along‑side significantly increased anal temperature,serum T3,T4,and CORT levels,cAMP/cGMP ratio,and immobility time in behavioral tests(P<0.01),as well as evident hippocampal pathological damage.Compared with the model group,all administration groups amelio‑rated these abnormalities to varying degrees(P<0.05 or P<0.01).Compared with the Radix Rehmanniae group,the 25％Fructus Ligustri Lucidi-processed Radix Rehmanniae group further improved physical signs,serum biochemical parameters,and behavioral per‑formance,up-regulated GR and BDNF protein levels(P<0.01),and alleviated hippocampal pathological injury.Conclusion Prepared by Fructus Ligustri Lucidi,the yin-nourishing,heat-clearing,and antidepressant effects of Radix Rehmanniae are enhanced.Its efficacy-enhancing mechanism may be associated with the up-regulation of GR and BDNF protein expression in the hippocampus.