加味小青龙汤对骨髓间充质干细胞移植后哮喘大鼠气道炎症的影响OA

Objective To investigate the mechanism by which Jiawei Xiaoqinglong Decoction(加味小青龙汤,JXD)alleviates airway inflammation in asthmatic rats after bone marrow mesenchymal stem cell(BMSC)transplantation.Methods Fifty SD rats were randomly divided into blank group,model group,BMSC transplantation model group,BMSC+JXD group,and BMSC+dexamethasone group.The asthmatic rat model was induced by ovalbumin(OVA)sensitization and challenge combined with cold air exposure.The BMSC transplantation model was established by injecting BMSCs via the tail vein on the basis of the asthmatic model.Subsequently,the rats in the treatment groups received JXD or dexamethasone,respectively,for one week.Asthma-related behavioral changes were recorded.Lung tissue pathology was observed via HE staining.The levels of IL-6 and IL-10 in bronchoalveolar lavage fluid(BALF)were detected by ELISA.The positive expression of CD86 and CD206 in lung tissue was assessed by immunofluorescence(IF)single staining.The protein expression levels of Toll-like receptor 4(TLR4)and nuclear factor-kappa B(NF-κB)pathway-related proteins were measured by Western blot(WB).Results Compared with the blank group,the asthma behavioral scores in the model group were significantly elevated(P<0.01).Pathological examination of lung tissues revealed extensive inflammatory cell infiltration,structural damage to alveoli and bronchi,and interstitial hyperplasia.The level of IL-6 in BALF was significantly increased,while the level of IL-10 was sig‑nificantly decreased(P<0.01).The relative fluorescence intensity of CD86 was markedly enhanced,whereas that of CD206 was signifi‑cantly reduced(P<0.01).The protein expression levels of TLR4 and phosphorylated NF-κB p65(p-NF-κB p65)were significantly upregulated(P<0.01).Compared with the model group,the BMSC transplantation model group,BMSC+JXD group,and BMSC+dexamethasone group demonstrated significant reductions in asthma behavioral scores(P<0.05).Inflammatory damage and structural disruption in lung tissues were alleviated in these groups.The level of IL-6 in BALF was decreased,while the level of IL-10 was signifi‑cantly increased(P<0.05,P<0.01).The fluorescence intensity of CD206 in lung tissues was markedly enhanced,whereas the protein expression of p-NF-κB p65 was significantly reduced(P<0.01,P<0.05).Both the BMSC+JXD and BMSC+dexamethasone groups showed significant decreases in CD86 fluorescence intensity and TLR4 protein expression(P<0.05).Compared with the BMSC trans‑plantation model group,both the BMSC+JXD group and the BMSC+dexamethasone group exhibited significantly reduced asthma behavioral scores(P<0.05).A further reduction in inflammatory cell infiltration and alleviation of alveolar structural damage were observed.The level of IL-6 in BALF decreased,while the level of IL-10 increased(P<0.05,P<0.01).The fluorescence intensity of CD86 markedly decreased,whereas that of CD206 increased(P<0.01,P<0.05).Conclusion JXD may balance macrophage polariza‑tion and alleviate airway inflammation in asthmatic rats after BMSC transplantation,which may be associated with its regulation of the TLR4/NF-κB signaling pathway.