中草药添加剂对乾华肉用美利奴公羊繁殖性能的影响OA

The experiment aimed to investigate the effects of different dietary supplementation levels of a Chinese herbal medicine additive on the semen quality and reproductive hormone secretion in breeding rams.A total of 40 Qianhua Mutton Merino rams aged two years were randomly divided into four groups,with five replicates per group and two rams per replicate.The control group was fed a basal diet,while experimental groups Ⅰ,Ⅱ,and Ⅲ were fed the basal diet supplemented with 0.5%,1.0%,and 1.5%of a self-formulated Chinese herbal medicine additive,respectively.Semen quality and plasma reproductive hormone levels were measured on the 0th,30th,60th and 90th days of the experiment.The pre-test period was seven days,and the formal test period was 90 days.The results showed that compared with the control group,on the 30th of the experiment,sperm density and sperm motility in groups Ⅰ,Ⅱ,and Ⅲ were significantly increased(P<0.05),while the sperm abnormality rate was significantly decreased(P<0.05).The levels of follicle-stimulating hormone(FSH),luteinizing hormone(LH),and testosterone(T)in both plasma and seminal plasma were significantly increased(P<0.05),and the activities of superoxide dismutase(SOD),glutathione peroxidase(GSH-Px),and catalase(CAT)in plasma were significantly enhanced(P<0.05).With the extension of feeding time,the ejaculate volume,sperm density,sperm motility,sperm acrosome integrity rate,reproductive hormone secretion levels,and antioxidant enzyme activities in the experimental groups were all increased,while the sperm abnormality rate exhibited a decreasing trend.The study shows that dietary supplementation with the Chinese herbal medicine additive can effectively improve the reproductive performance of Qianhua Mutton Merino rams.Based on a comprehensive evaluation of all measured parameters,the 1.5%supplementation level exhibits a relatively superior effect.