益肾活血助孕汤对多囊卵巢综合征不孕症大鼠卵巢颗粒细胞Ca2+浓度、细胞凋亡及生殖功能的影响OA

Objective To observe the effect of Yishen Huoxue Zhuyun Decoction on infertile rats with polycystic ovary syndrome(PCOS)and its effect on ovarian granulosa cell apoptosis and calcium concentration.Methods Seventy-five SPF-grade female SD rats were selected,with 15 assigned to a control group and the other 60 for modeling.Another 25 SPF-grade male SD rats were later introduced for co-housed mating.A PCOS model was established by letrozole administration via gavage.After successful modeling,the model rats were divided into a model group,a low-dose Yishen Huoxue Zhuyun Decoction group(YSHX-L group),a high-dose Yishen Huoxue Zhuyun Decoction group(YSHX-H group),and a metformin group(MET group)using the random number table method,with 15 rats in each group.The model group and the control group were administered normal saline via gavage,while the MET group was administered 300 mg/kg metformin via gavage.The YSHX-L group and the YSHX-H group were administered 0.48 and 0.96 g/kg of the Decoction via gavage,respectively.The intervention was performed once daily for 28 days.Afterwards,serum follicle-stimulating hormone(FSH),luteinizing hormone(LH),estradiol(E2),and testosterone(T)levels were detected by enzyme-linked immunosorbent assay(ELISA).The pathological changes of ovarian tissues were determined via hematoxylin and eosin(HE)staining.The ultrastructural changes of ovarian tissues were observed using transmission electron microscopy.Ovarian granulosa cell apoptosis and calcium concentration were measured by flow cytometry.Furthermore,male and female rats in the remaining groups were co-housed for mating at a ratio of 2∶1 to observe the pregnancy success rate.Results Compared with the blank group,the model group exhibited decreased serum E2 level and calcium concentration of ovarian granulosa cells,increased serum T,FSH,and LH levels,and ovarian granulosa cell apoptosis rate,significant follicular atresia,reduced number of ovarian granulosa cells,damaged cellular morphology and abnormal mitochondrial structure,accompanied by a declined pregnancy success rate(P<0.05).Compared with the model group,the YSHX-H group and the MET group showed elevated serum E2 levels and ovarian granulosa cell calcium concentration,decreased serum T,FSH,and LH levels,and ovarian granulosa cell apoptosis,alleviated damage of ovarian pathology and granulosa cell ultrastructure,recovered mitochondrial morphology and quantity,alongside an increased pregnancy success rate(P<0.05).No statistically significant difference was reported between the YSHX-L group and the model group in any indicator(P>0.05).Compared with the YSHX-H group,the MET group demonstrated lower serum E2 levels and ovarian granulosa cell calcium concentration,elevated serum T,FSH,and LH levels,and ovarian granulosa cell apoptosis,as well as a reduced pregnancy success rate(P<0.05).Conclusion Yishen Huoxue Zhuyun Decoction exerts a positive effect on PCOS infertility.It improves infertility symptoms by regulating ovarian granulosa cell calcium concentration,estrogen level,and ovarian granulosa cell apoptosis to a certain extent.