银耳多糖对小鼠运动疲劳恢复和抗氧化作用研究OA

To investigate the effects of intragastric gavage of Tremella fuciformis polysaccharides(TFPs)on exercise fatigue recovery and antioxidant capacity in mice,sixty male Kunming mice were randomly divided into five groups with 12 mice in each group:control group(no exercise training+intragastric gavage of normal saline),training group(exercise training+intragastric gavage of normal saline),low-dose TFP group(exercise training+intragastric gavage of TFPs at 150 mg/kg BW),medium-dose TFP group(exercise training+intragastric gavage of TFPs at 300 mg/kg BW),and high-dose TFP group(exercise training+intragastric gavage of TFPs at 600 mg/kg BW).After 21 consecutive days of gavage treatment,the contents of hepatic glycogen and muscle glycogen were detected.Meanwhile,the activities of superoxide dismutase(SOD),as well as the contents of malondialdehyde(MDA)and glutathione(GSH)in the liver,myocardium and gastrocnemius muscle of mice were measured.The results showed that compared with the control group,the levels of hepatic glycogen and muscle glycogen in the training group decreased significantly(P<0.05),while those in the medium-and high-dose TFP groups significantly increased(P<0.05).Compared with the training group,the hepatic SOD activity and GSH content were significantly elevated,and the hepatic MDA content was significantly reduced in the medium-and high-dose TFP groups(P<0.05).For myocardium tissue,SOD activity and GSH content were significantly enhanced in the medium-and high-dose TFP groups,whereas only the high-dose TFP group exhibited a significant reduction in myocardial MDA content(P<0.05).In gastrocnemius muscle,SOD activity was significantly improved in all low-dose,medium-dose and high-dose TFP groups;GSH content was significantly increased only in the medium-dose TFP group,and MDA content decreased significantly merely in the low-dose TFP group(P<0.05).In conclusion,TFPs can alleviate exercise-induced glycogen consumption,improve the antioxidant capacity of the liver,myocardium and skeletal muscle,mitigate lipid peroxidation damage,and effectively accelerate exercise fatigue recovery in mice.Moreover,medium and high doses of TFPs present better overall efficacy.