木芙蓉叶总黄酮的提取工艺研究OA

This experiment aimed to improve the extraction rate of total flavonoids from Hibiscus mutabilis leaf.Ultrasound-assisted combined with ultraviolet spectrophotometry was used to analyze the total flavonoids content in Hibiscus mutabilis leaf.Key variables were screened through single-factor experiments,and the extraction process was optimized using response surface methodology.The results showed that the ethanol volume fraction of 74％,liquid-to-material ratio of 48 mL·g-1,extraction temperature of 53℃,and ultrasonic time of 20 min were the optimal extraction process for total flavonoids from Hibiscus mutabilis leaf,and the extraction rate was 29.37 mg·g-1.This experiment showed that the ultrasonic-assisted extraction method is simple to operate,short in extraction time,stable in extraction efficiency,and green and environmentally friendly,providing a scientific basis for the extraction process of flavonoids in Hibiscus mutabilis leaf and their comprehensive development and utilization of resources.