玉竹根尖离体培养及芽增殖技术优化研究OA
Optimization of In Vitro Root Tip Culture and Shoot Multiplication Technology for Polygonatum odoratum(Mill.)Druce
本研究以玉竹幼嫩根尖为外植体,系统分析灭菌条件、外植体部位、低温预处理、植物生长调节剂配比、蔗糖浓度及培养基 pH 值对离体培养效果的影响.结果表明:75％乙醇 8s 结合 0.1％升汞 4min 可获得较好的灭菌效果;长度约 0.5cm 的根尖中段具有较高的不定芽诱导能力;4℃低温预处理 6d 可促进芽分化;MS 培养基添加 6-BA 0.7mg·L-1和 NAA 0.6mg·L-1时芽增殖效果较优;蔗糖浓度 25g·L-1、培养基 pH 5.8 有利于芽的正常生长.本研究建立了适用于玉竹根尖外植体的离体培养及芽增殖技术体系,为玉竹种苗快速繁殖提供技术参考.
In this study,young root tips of Polygonatum odoratum(Mill.)Druce were used as explants.The effects of sterilization conditions,explant segments,low-temperature pretreatment,combinations of plant growth regulators,sucrose concentration and medium pH on in vitro culture performance were systematically investigated.The results showed that the optimal sterilization procedure was 75％ethanol treatment for 8 s followed by 0.1％mercuric chloride treatment for 4 min.The middle segments of root tips with a length of about 0.5 cm exhibited a high capacity for adventitious bud induction.Low-temperature pretreatment at 4℃for 6 days significantly promoted bud differentiation.The MS medium supplemented with 0.7 mg·L-1 6-BA and 0.6 mg·L-1 NAA achieved the best shoot proliferation efficiency.A sucrose concentration of 25 g·L-1 and medium pH of 5.8 were conducive to normal bud growth.This study established an efficient in vitro culture and shoot proliferation system using root tip explants of Polygonatum odoratum(Mill.)Druce,which provides a technical reference for the rapid propagation of Polygonatum odoratum(Mill.)Druce seedlings.
吕娇娇;熊胜;刘明新
湖南农业大学生科学与技术学院,长沙 410125湖南农业大学生科学与技术学院,长沙 410125湖南农业大学生科学与技术学院,长沙 410125||岳麓山实验室,长沙 410128
农业科技
玉竹根尖组织培养不定芽诱导快速繁殖
Polygonatum odoratum(Mill.)Druceroot tiptissue cultureadventitious bud inductionrapid propagation
《农业与技术》 2026 (10)
1-6,6
国家新农村发展研究院植物资源开发项目(项目编号:xczx-2023175)
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