SKA3蛋白在子宫颈癌的组织和HeLa细胞系中的表达及对JAK/STAT信号通路的影响OA
Expression of SKA3 protein in cervical cancer tissues and HeLa cell lines and its effect on JAK/STAT signaling pathway
目的 探讨纺锤体与着丝粒相关蛋白3(spindle and centromere-associated protein 3,SKA3)在子宫颈癌组织中的表达及临床病理联系,在子宫颈癌HeLa细胞SKA3表达下调后细胞增殖、迁徙改变,及对Janus激酶/信号转导与转录激活因子(JAK/STAT)信号通路在其中的作用相关蛋白表达影响.方法 免疫组化法检测69例子宫颈癌组织中SKA3蛋白表达并探讨与临床病理联系.体外实验中,采用脂质体Lipofectamin 2000转染SKA3-siRNA子宫颈癌HeLa细胞,转染后,SKA3蛋白表达改变采用免疫细胞荧光法和Western blot法观察,HeLa细胞SKA3-siRNA 干扰后,增殖影响采用MTT法与细胞克隆形成实验观察,JAK/STAT信号通路相关蛋白改变采用Western blot法检测.结果 与子宫颈癌旁组织相比,SKA3蛋白阳性在子宫颈癌组织中显著增强,且以FIGO分期中ⅠB2~ⅡA1期(P=0.021 8)、低分化者(P=0.017)、有淋巴结转移(P=0.010 1)等子宫颈癌预后较差的情况下的SKA3蛋白表达更为显著;子宫颈癌HeLa细胞免疫细胞荧光法和Western blot法均证实转染SKA3-siRNA后SKA3蛋白表达下降,增殖能力降低,克隆形成数目也显著下降,且转染后p-JAK、p-STAT蛋白表达也明显减低(P<0.05).结论 在子宫颈癌组织中存在SKA3蛋白阳性增强,且反映预后较差;SKA3-siRNA 干扰后可降低子宫颈癌HeLa细胞的增殖,且与降低JAK/STAT信号通路相关蛋白有关.
Objective To explore the expression of spindle and centromere-associated protein 3(SKA3)in cer⁃vical cancer tissues and its clinicopathological correlations,as well as the changes in cell proliferation and migration after the down-regulation of SKA3 expression in cervical cancer HeLa cells,and its effects on the expression of pro⁃teins related to the Janus kinase/signal transducer and activator of transcription(JAK/STAT)signaling pathway.Meth⁃ods The expression of SKA3 protein in 69 cases of cervical cancer tissues was detected by immunohistochemistry and its clinicopathological correlations were analyzes.In vitro experiments,SKA3-siRNA cervical cancer HeLa cells were transfected with Lipofectamin 2000.After transfection,the expression changes of SKA3 protein were observed by im⁃munofluorescence and Western blotting.After SKA3-siRNA interference in HeLa cells,the proliferation effect was ob⁃served by MTT assay and cell clone formation experiment,and the changes of JAK/STAT signaling pathway-related proteins were detected by Western blotting.Results Compared with the adjacent tissues of cervical cancer,the posi⁃tive expression of SKA3 protein was significantly enhanced in cervical cancer tissues,and the expression of SKA3 pro⁃tein was more significant in cases with poor prognosis of cervical cancer such as stage ⅠB2-ⅡA1in FIGO(P=0.021 8),poor differentiation(P=0.017),and lymph node metastasis(P=0.010 1).Both immunofluorescence assay and Western blotting confirmed that after HeLa cells transfection with SKA3-siRNA,the expression of SKA3 protein de⁃creased,the proliferation ability decreased,the number of clone formations decreased significantly,and the expres⁃sions of p-JAK and p-STAT proteins also decreased significantly after transfection(P<0.05).Conclusion There is an enhanced positive expression of SKA3 protein in cervical cancer tissues,and it reflects a poor prognosis.SKA3-siRNA interference can reduce the proliferation of cervical cancer HeLa cells and is related to the reduction of proteins associated with the JAK/STAT signaling pathway.
孙传名;吴莉莉;田静;陈苏环;张梦妍;郁玮志;邵玉宝;王琦;陈晓宇
合肥市第三人民医院 妇产科,合肥 230022合肥市第三人民医院 病理科,合肥 230022合肥市第三人民医院 病理科,合肥 230022安徽医科大学组织胚胎学教研室,合肥 230032安徽医科大学组织胚胎学教研室,合肥 230032安徽医科大学组织胚胎学教研室,合肥 230032安徽医科大学组织胚胎学教研室,合肥 230032安徽省立医院病理科,合肥 230036安徽医科大学组织胚胎学教研室,合肥 230032
医药卫生
子宫颈肿瘤SKA3JAK/STAT信号通路细胞增殖临床病理特征预后
cervical neoplasmsSKA3JAK/STAT signaling pathwaycell proliferationclinicopathological charac⁃teristicsprognosis
《临床与实验病理学杂志》 2026 (9)
1172-1177,1184,7
合肥市第三人民医院科研项目(SYKY202401)、合肥市卫生健康科研项目(Hwk2025zc011)、安徽医科大学临床医学("5+3"一体化)专业"早期接触科研"训练计划项目(2025-ZQKY-036) Scientific Research Project of Hefei Third People's Hospital(SYKY202401)Hefei Health and Wellness Research Project(Hwk2025zc011)Anhui Medical University Clinical Medicine Program("5+3"Inte⁃grated Curriculum)"Early Exposure to Scientific Research"Training Program(2025-ZQKY-036)
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