乳腺癌中SMAP1的表达及其对肿瘤细胞生物学行为的影响OA

Objective To investigate the expression of stromal membrane-associated protein 1(SMAP1)in breast cancer and determine its potential role in modulating malignant phenotypes of tumor cells.Methods SMAP1 mRNA and protein expression in breast cancer and adjacement normal breast tissues was analyzed using the GEPIA3 online database and immunohistochemistry,respectively.The correlation between SMAP1 expression and clinico⁃pathological characteristics and overall survival of breast cancer patients was evaluated by using the Kaplan-Meier Plot⁃ter database and immunohistochemistry analysis,respectively.In vitro experiments,the impact SMAP1 expression on breast cancer cell behaviors were assessed using CCK-8 assay,colony formation,wound healing,and Transwell migra⁃tion and invasion assays.Results Both bioinformatic data and immunohistochemical(IHC)staining indicated a marked elevation of SMAP1 in breast cancer specimens compared with adjacement normal breast tissues(mRNA ex⁃pression was approximately 1.21-fold than that in normal tissues,Log2FC=0.28,P<0.05).Notably,IHC results showed a significantly greater proportion of high SMAP1 expression in carcinomas(52.8%)versus adjacement normal breast tissues(28.3%).Clinicopathological correlation analysis demonstrated that SMAP1 overexpression was signifi⁃cantly positively correlated with tumor diameter exceeding 2 cm(P=0.008),lymph node metastasis(P=0.001),ad⁃vanced TNM staging(P=0.034),and an elevated Ki67 index(P=0.009).Prognostically,patients in the high-SMAP1 group had significantly shorter overall survival than those with low expression(HR=1.40,95%CI:1.26~1.57,P<0.05).To explore biological functions,we utilized Western blotting to validate the silencing efficiency of SMAP1 in MCF-7 cells.In vitro experiments subsequently confirmed that silencing SMAP1 suppressed the prolifera⁃tion of MCF-7 cells,accompanied by a decreased number of colonies(P<0.05);moreover,the wound healing rate and the numbers of migrated and invaded cells in Transwell assays were significantly reduced compared with the con⁃trol group(P<0.05).Conclusion SMAP1 may act as an oncogene in breast cancer,participating in tumor malignant progression,and may serve as a potential biological marker.