电子束辐照对西藏木瓜粉灭菌效果及品质的影响OA

To investigate the sterilization efficacy and quality impact of electron beam irradiation on Chaenomeles thibetica powder,this study employed irradiation doses of 0(CK),2,4,6,8,10 kGy electron beam irradiation doses.The study compared irradiation effects on microbial counts,textural characteristics,thin-layer chromatograms,total polysaccharide content,polysaccharide fingerprint patterns,total oleanolic acid and ursolic acid content,and their corresponding fingerprint patterns.Results indicated that compared with the CK group,all electron beam irradiation doses significantly reduced the total counts of aerobic bacteria,molds,and yeasts in Chaenomeles thibetica powder.Furthermore,the control bacterium Escherichia coli was not detected before or after irradiation.At an initial total aerobic bacteria count of 2.2×10³(CFU·g-¹)and total mold and yeast count of 9.8×10³(CFU·g-¹)in Chaenomeles thibetica powder,2 kGy irradiation dose brought microbial levels into compliance with the requirements of the Pharmacopoeia of the People's Republic of China,2020 Edition,Volume Ⅳ.Compared to 0 kGy irradiation,electron beam doses ranging from 2 to 10 kGy showed no significant effects on the physical properties,thin-layer chromatograms,total polysaccharide content,polysaccharide fingerprint patterns(similarity>0.998),total oleanolic acid and ursolic acid content,or their corresponding fingerprint patterns(similarity>0.999)of Chaenomeles thibetica powder.In summary,within the established irradiation dose range,different electron beam irradiation doses can ensure the quality of Chaenomeles thibetica powder,with the excellent sterilization efficacy achieved at 2 kGy.This study provides a theoretical basis for the application of electron beam irradiation in the sterilization process of Chaenomeles thibetica.