兰州百合SWEET基因家族鉴定及其在鳞茎形成过程中的表达分析OA
Genome-Wide Identification of SWEET Gene Family in Lilium davidii var.willmottiae and Expression Analysis during Bulblet Formation
糖外排转运蛋白(sugar will eventually be exported transporters,SWEETs)是近年来在植物中发现的一类糖转运蛋白,在植物生长发育和胁迫响应等多种过程中发挥重要作用.为了探究该家族基因对兰州百合(Lilium davidii var.willmottiae)小鳞茎形成的影响,本研究基于全基因组对兰州百合LdwSWEETs基因家族进行鉴定,并对其进行全面的生物信息分析,包括系统发育、染色体定位、保守结构域及顺式调控元件等分析,同时开展了小鳞茎形成过程不同时期的LdwSWEETs表达模式及部分基因的组织表达模式分析.本研究鉴定出兰州百合共有37个LdwSWEETs基因,不均匀地分布在9条染色体上;顺式调控元件分析显示,LdwSWEETs启动子上含有多个光、植物生长发育和植物激素调控相关的元件,其表达可能受光、生长素、脱落酸和茉莉酸的调控.实时荧光定量PCR(qRT-PCR)结果表明,不同LdwSWEET 基因存在多种表达模式,其中 LdwSWEET29 在 S1 时期显著上调表达而 LdwSWEET2 和LdwSWEET33在S2时期显著上调表达,三者的上调倍数均达10倍以上.不同基因组织特异性表达结果显示LdwSWEET1在花药中显著表达,LdwSWEET33在地下器官中的基生根和鳞茎中显著表达.本研究系统地揭示了兰州百合SWEETs基因家族成员的结构特征,阐明了其成员在小鳞茎形成过程中及不同组织的表达规律,并筛选出LdwSWEET1、LdwSWEET2、LdwSWEET29和LdwSWEET33等关键候选基因,为深入了解兰州百合鳞茎形成的调控机制,以及鳞茎产量的优化提供了基因资源.
The SWEETs(sugar will eventually be exported transporters)constitute a family of sugar transporters newly identified in plants,which play pivotal roles in diverse biological processes including plant growth,development,and responses to abiotic and biotic stresses.To investigate the roles of SWEET genes during bulblets formation in Lanzhou lily(Lilium davidii var.willmottiae),the LdwSWEET gene family was characterized at the whole-genome level,followed by comprehensive bioinformatics analyses,including phylogeny,chromosome distribution,conserved domain and cis-regulatory element etc.Spatio-temporal expression patterns were further examined using quantitative real-time polymerase chain reaction(qRT-PCR).The results showed that a total of 37 LdwSWEETs genes were identified and found to be unevenly distributed across 9 chromosomes;cis-regulatory element analysis revealed that the promoters of LdwSWEET genes harbored numerous elements related to light responsiveness,plant growth and development,and phytohormone regulation,implying that their transcription may be modulated by light,auxin,abscisic acid and jasmonic acid.Moreover,distinct expression patterns were observed among LdwSWEET members:LdwSWEET29 exhibited a marked upregulation at the S1 stage,while LdwSWEET2 and LdwSWEET33 were sharply elevated at the S2 stage,with relative expression fold changes all exceeding 10-fold.Tissue-specific expression analysis revealed that LdwSWEET1 was predominantly expressed in anthers,whereas LdwSWEET33 was highly abundant in underground vegetative organs such as basal roots and bulbs.This study systematically uncovered the structural features of the LdwSWEET gene family and delineated their dynamic expression patterns during bulblet formation and across various tissues.Several key candidate genes such as LdwSWEET1,LdwSWEET2,LdwSWEET29,and LdwSWEET33 were identified,providing important genetic resources for further dissection of the regulatory network underlying bulblet formation and improving bulb yield in Lanzhou lily.
邹纪文;康婕;杨中振;凌玲;李召雷;秦美珠;夏宜平;吴昀
浙江理工大学建筑工程学院风景园林系,浙江 杭州 310018浙江农林大学园艺学院百合发育生物学与种质创新实验室,浙江 杭州 311300浙江农林大学园艺学院百合发育生物学与种质创新实验室,浙江 杭州 311300浙江农林大学园艺学院百合发育生物学与种质创新实验室,浙江 杭州 311300浙江农林大学园艺学院百合发育生物学与种质创新实验室,浙江 杭州 311300浙江农林大学园艺学院百合发育生物学与种质创新实验室,浙江 杭州 311300浙江大学农业与生物技术学院观赏植物基因组与基因工程实验室,浙江 杭州 310058浙江理工大学建筑工程学院风景园林系,浙江 杭州 310018||浙江农林大学园艺学院百合发育生物学与种质创新实验室,浙江 杭州 311300
农业科技
兰州百合糖外排转运蛋白基因家族鉴定鳞茎发生基因表达分析
Lilium davidii var.willmottiaesugar will eventually be exported transportersgene family identificationbulblet initiationgene expression analysis
《核农学报》 2026 (11)
2165-2176,12
国家自然科学基金面上项目(32372743),国家自然科学基金青年科学基金项目(32002071),浙江农林大学科研启动经费(2024LFR125),2022年杭州市农业与社会发展科研引导项目(20220919Y1)
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