水稻白色条纹叶突变体wsl10-t的表型鉴定与基因定位OA

Leaf color mutants are important genetic materials for functional studies of the photosynthetic system and high-yield breeding in rice.A stably inherited vein-albino mutant wsl10-t was obtained through heavy-ion beam irradiation of the cultivar Wuyunjing 7.Its phenotype,chlorophyll content,and photosynthetic rate were characterized,and genetic analysis,gene mapping,candidate gene analysis were performed to investigate the mechanism regulating leaf color in wsl10-t.Compared with the wild type(WT),the mutant wsl10-t showed progressive whitening of the leaf midrib starting from the seedling stage,along with significant reductions in transpiration rate,net photosynthetic rate,and photosynthetic pigments content.At maturity,the mutant displayed markedly decreased flag leaf length and width,effective tiller number,seed setting rate,grain length and yield per plant,while grain width and thickness were significantly higher than those of the wild type.Genetic analysis revealed that the mutant phenotype is controlled by a single recessive nuclear gene.The gene was mapped to a 7.1-kb region on chromosome 4,which contains four putative open reading frames.Sequencing of the candidate gene identified a single base substitution in Os04g0601800 within exon 3,resulting in the replacement of a glycine(Gly)residue with an aspartate(Asp)residue.This mutation represents a novel multiple allele of WSP1.These findings provide a theoretical basis and experimental materials for further research on leaf color mutations associated with aberrant chloroplast RNA editing.