毛白杨CHS基因家族鉴定与表达分析OA

[Objective]To identify the chalcone synthase(CHS)gene family members in Populus tomentosa and explore the molecular mechanism of anthocyanin accumulation in its leaves under strong light induction,providing basic information for the improvement of stress resistance and the creation of new varieties of P.tomentosa.[Method]By analyzing the whole genome data of P.tomentosa,12 CHS family members were identified.Their evolutionary relationships,protein structures and physico-chemical properties,gene structures and conserved motifs,promoter cis-acting elements,gene tissue expression patterns,and changes in gene expression levels under strong light treatment were studied.[Result]The 12 PtoCHSs were distributed on 6 chromosomes.Except for PtoCHS2A which contained 3 exons,the other 11 family members contained 2 exons.Members with close evolutionary relation-ships showed similar gene structures and conserved motif compositions.The PtoCHSs genes had diffe-rent expression patterns,and their gene promoter regions contained multiple elements responding to plant hormones,light,drought,low temperature and other stress signals,indicating that PtoCHS genes have diverse functions in the growth and development of P.tomentosa and its adaptation to the environment.Except for PtoCHS1D and PtoCHS6A,the other 10 PtoCHSs responded to strong light induction,among which PtoCHS6D had the largest relative change,suggesting that it is closely related to the accumulation of anthocyanins and the reddening of P.tomentosa leaves under strong light induc-tion.Subcellular localization experiments confirmed that PtoCHS6D was located in the endoplasmic reticulum.[Conclusion]The characteristics and expression patterns of the PtoCHS gene family in P.tomentosa were analyzed.The sequences of its family members were relatively conserved,and their functions were diverse.The expression abundance and preference characteristics of PtoCHS2A,PtoCHS3A,PtoCHS3D and PtoCHS6D in roots or leaves suggested that these genes have important bio-logical functions in the development of roots and leaves.