白花泡桐PfHBs基因家族鉴定及响应植原体侵染的免疫应答分析OA

[Objective]To identify the members of the homeobox(HB)gene family in the P.fortunei genome and investigate the regulatory role of this gene family during the development of Paulownia witches'broom(PaWB).[Method]Members of the PfHBs gene family were identified through bioin-formatics analysis.Their physicochemical properties,phylogenetic relationships,intra-and interspe-cific collinearity,and promoter cis-acting elements were systematically analyzed.Protein-protein inter-actions were predicted using String and AlphaFold3.Furthermore,transcriptome sequencing from P.fortunei before and after phytoplasma infection were integrated to identify,PfHBs family,members associated with the development of witches'broom disease.[Result]A total of 121 PfHBs gene family members were identified in P.fortunei.Evolutionary analysis revealed that these genes were primarily originated from segmental and tandemduplication events and could be classified into 10 subfamilies.Gene expression analysis and subcellular localization analyses revealed that PfHB94 was highly expressed in stems and encode a protein localized to the nucleus,suggesting that it may be associated with the early immune response triggered by PaWB.Protein-protein interaction analysis,molecular docking,and yeast two-hybrid experiments confirmed an interaction between PfHB94 and PfHB98.[Conclusion]A total of 121 members were identified in the PfHB gene family of P.fortunei.The inter-action between PfHB94 and PfHB98 may regulate the expression of salicylic acid biosynthesis-related genes,thereby triggering an immune response against phytoplasma infection.