三七总皂苷调节HMGA1/Wnt/β-catenin信号通路对股骨骨折大鼠骨愈合的影响OA
Effects of Panax notoginseng saponins on bone healing in rats with femo-ral fracture by regulating the HMGA1/Wnt/β-catenin signaling pathway
目的:探讨三七总皂苷(PNS)对股骨骨折大鼠骨愈合及HMGA1/Wnt/β-catenin信号通路的影响.方法:48只股骨骨折模型大鼠分为模型组、L-PNS组(100 mg/kg PNS灌胃)、H-PNS组(200 mg/kg PNS灌胃)、H-PNS+si-HMGA1 组(200 mg/kg PNS 灌胃+尾静脉注射 0.75 μmol/kg si-HMGA1),每组 12 只,1 次/d,给药 6 d.给药结束,拍摄X射线片观察骨痂形成情况;生物力学检测评估股骨最大负荷;HE染色观察股骨组织形态,番红O-固绿染色检测软骨组织形态;RT-qPCR检测大鼠骨折断面组织中Runt相关转录因子2(Runx2)、成骨细胞特异性转录因子(Osterix)、骨钙素(Osteocalcin)mRNA 的表达;Western blot 检测骨折断面组织中 Runx2、Osterix、Osteocalcin、HMGA1、Wnt、β-catenin蛋白表达.结果:与模型组相比,L-PNS组和H-PNS组骨折处残留编织骨较少,成骨细胞较多,骨痂形成评分,股骨最大载荷,Runx2、Osterix、Osteocalcin mRNA 和蛋白表达及 HMGA1、Wnt、p-β-catenin/β-cate-nin蛋白表达均升高(P＜0.05),且H-PNS组高于L-PNS组(P＜0.05);与H-PNS组相比,H-PNS+si-HMGA1组大鼠骨痂处于重建阶段,有各种残留编织骨,骨痂形成评分,股骨最大载荷,Runx2、Osterix、Osteocalcin mRNA和蛋白表达以及HMGA1、Wnt、p-β-catenin/β-catenin蛋白表达均降低(P＜0.05).结论:PNS可促进股骨骨折大鼠骨愈合,其机制可能与激活HMGA1/Wnt/β-catenin通路有关.
Aim:To investigate the effects of panax notoginseng saponins(PNS)on bone healing and the HMGA1/Wnt/β-catenin signaling pathway in rats with femoral fracture.Methods:Forty-eight rats with femoral fracture were divided into the model group,L-PNS group(intragastric administration of 100 mg/kg PNS),H-PNS group(intragastric administra-tion of 200 mg/kg PNS),and H-PNS+si-HMGA1 group(intragastric administration of 200 mg/kg PNS combined with tail-vein injection of 0.75 μmol/kg si-HMGA1),with 12 rats in each group.The drugs were administered once daily for 6 days.After administration,X-ray examination was performed to observe callus formation.Biomechanical testing was used to assess the maximum load of the femur.HE staining was applied to observe femoral tissue morphology,and safranin O-fast green staining was used to detect cartilage tissue morphology.RT-qPCR was used to detect mRNA expression of runt-related tran-scription factor 2(Runx2),osteoblast-specific transcription factor(Osterix)and Osteocalcin in fracture tissue.Western blot was performed to detect the protein expression of Runx2,Osterix,Osteocalcin,HMGA1,Wnt and β-catenin at the fracture site.Results:Compared with the model group,the L-PNS and H-PNS groups showed less residual woven bone and more os-teoblasts at the fracture site.The callus formation score,femoral maximum load,mRNA and protein expressions of Runx2,Osterix and Osteocalcin,as well as protein expressions of HMGA1,Wnt and p-β-catenin/β-catenin were all increased(P＜0.05),and the H-PNS group showed higher levels than the L-PNS group(P＜0.05).Compared with the H-PNS group,the H-PNS+si-HMGA1 group presented callus in the remodeling stage with abundant residual woven bone;the callus formation score,femoral maximum load,mRNA and protein expressions of Runx2,Osterix and Osteocalcin,and protein expressions of HMGA1,Wnt and p-β-catenin/β-catenin were all decreased(P＜0.05).Conclusion:PNS can promote bone healing in rats with femoral fracture,and its mechanism may be related to the activation of the HMGA1/Wnt/β-catenin signaling pathway.
黄振明;吴飞;汪建雄;曹园
武汉市第九医院创伤外科 武汉 430000武汉大学人民医院骨科 武汉 430000武汉市第九医院创伤外科 武汉 430000湖北文理学院附属襄阳市中心医院骨科 湖北襄阳 441021
医药卫生
三七总皂苷股骨骨折骨愈合HMGA1/Wnt/β-catenin信号通路大鼠
Panax notoginseng saponinsfemoral fracturebone healingHMGA1/Wnt/β-catenin signaling pathwayrat
《郑州大学学报（医学版）》 2026 (5)
21-25,5
湖北省科技计划自然科学基金项目(2020HBA216)
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