心阴片调控PPARγ/PI3K/AKT/eNOS通路改善肺动脉高压的作用机制OA

Objective To explore the mechanism of Xinyin tablets in improving pulmonary hypertension by regulating the PPARγ/PI3K/AKT/eNOS signaling pathway.Methods A total of 45 SD rats were randomly divided into control group(Control),model group(MCT),Sildenafil group(Sildenafil),low-dose Xinyin tablets group(XYP-L),and high-dose Xinyin tablets group(XYP-H).Pulmonary hypertension rat model was established by Monocrotaline induction.Interventions were started on the second day after modeling,each group was administered the corresponding intervention for 3 consecutive weeks.Right heart catheterization was used to measure right ventricular systolic pressure(RVSP),HE staining was used to evaluate pulmonary vascular remodeling,TUNEL assay was used to detect pulmonary vascular cell apoptosis.Enzyme-linked immunosorbent assay was used to detect the level of endothelin-1(ET-1)in lung tissue.Western blotting was used to detect the expression levels of PPARγ,PI3K,AKT,eNOS proteins in lung tissue.Results Compared with the MCT group,the XYP-H group exhibited markedly reduced RVSP and RVHI(P<0.01),significantly decreased WT%and WA%(P<0.01),as well as downregulated ET-1 expression(P<0.01).The protein expression levels of PPARγ,PI3K,p-AKT,AKT,eNOS and the Bax/Bcl-2 ratio in lung tissue were elevated(P<0.01),while PCNA protein expression was significantly downregulated(P<0.01).The pulmonary vascular cell apoptosis index was notably increased(P<0.01).Conclusion Xinyin tablets could alleviate pulmonary arterial hypertension by intervening in the PPARγ/PI3K/AKT/eNOS signaling pathway,dilating pulmonary arteries,and improving pulmonary vascular remodeling.