双金属协同增强Fe/Cu-MOF纳米酶的构建及其对四环素和苯酚的高灵敏检测OA

Designing and synthesizing bimetallic nanozymes with multiple enzyme-mimicking activities is a key research focus in the field of environmental analysis.In this study,a heterogeneous bimetallic Fe/Cu metal-organic framework(MOF)nanozyme with excellent oxidase(OXD)-like and laccase-like dual catalytic activities was successfully constructed via a one-pot self-assembly of Fe3+and Cu2+with 4,4′-biphenyldicarboxylic acid(4,4′-BPDC).Mechanistic studies demonstrated that the synergistic interaction between Fe and Cu active sites within the MOF framework effectively improved electron-transfer efficiency and substrate affinity.Moreover,the dual redox cycles of Fe3+/Fe2+and Cu2+/Cu+effectively promoted the in situ generation of reactive oxygen species(ROS),particularly superoxide anion radicals(·O2–).Based on these remarkable properties,an efficient detection platform for environmental pollutants was developed,enabling highly sensitive and selective detection of tetracycline(TC)and phenol under neutral conditions,with limits of detection(LODs)as low as 1.25 μmol/L and 2.51 μmol/L,respectively.In spiked recovery experiments in real water samples,the proposed system achieved recoveries ranging from 94.1％to 103.0％,with relative standard deviations(RSDs)of less than 5％.This study not only provided new insights into the rational design of heterogeneous bimetallic MOF nanozymes but also offered a feasible analytical strategy for the on-site detection of pollutants and water quality assessment in complex environmental water bodies.