基于Apriori算法、Phi系数与Ward.D聚类的中药治疗排卵障碍性不孕症排卵期用药规律及作用机制研究OA

OBJECTIVE:To explore the medication rules and potential mechanism of action of traditional Chinese medicine(TCM)in the treatment of ovulatory disorder infertility(ODI)during the ovulatory phase based on the Apriori algorithm,Phi coefficient and Ward.D clustering.METHODS:Clinical literature on TCM in the treatment of ODI was retrieved from CNKI,Wanfang and PubMed databases.Standardized prescriptions were entered,and R software was used to perform frequency analysis on the herbs and their four natures,five flavors and channel tropism.Association rules were mined,and hierarchical cluster analysis was conducted using the Ward.D algorithm.Finally,co-occurrence analysis was used for visualization.The core prescriptions and potential targets for the treatment of ODI were screened,and protein-protein interaction analysis as well as GO and KEGG enrichment analyses were performed.Molecular docking technology was used to evaluate the binding affinity and stability between the main active ingredients and their corresponding targets.RESULTS:A total of 157 TCM formulas for the treatment of ODI were screened,involving 190 herbs.High-frequency herbs included Angelicae sinensis,Cuscutae chinensis and Rehmanniae glutinosa,etc.The natures and flavors of the herbs were mainly warm,sweet,and attributed to the liver and kidney meridians.The core prescription consisted of A.sinensis,C.chinensis,R.glutinosa,Dioscoreae opposita and Cornius officinalis.The active ingredients of the core prescription,including sitosterol,stigmasterol,isofucosterol,β-sitosterol,hyperoside,etc.,mainly acted on key targets such as CXCL8 to regulate cancer pathways,chemical carcinogenesis-receptor activation,endocrine resistance,estrogen regulation and other signaling pathways,and exhibited favorable binding affinity and stability with the key target CXCL8.CONCLUSIONS:The compatibility of the core prescription used in the TCM treatment of ODI is derived from the classical prescription Yougui pill.Its main components can improve the overall functional state and promote ovulation by regulating cancer pathways,chemical carcinogenesis-receptor activation,endocrine resistance,estrogen regulation and other signaling pathways.