香青藤乙酸乙酯部位化学成分及其体外抗肿瘤、抗氧化活性研究OA

AIM To study the chemical constituents from the ethyl acetate fraction of Illigera aromatica S.Z.Huang et S.L.Mo and their in vitro anti-tumor,anti-oxidant activities.METHODS Separation and purification were performed using silica gel,Sephadex LH-20,reverse-phase ODS column chromatography and semi-preparative HPLC,then the structures of obtained compounds were identified by physicochemical properties and spectral data.The inhibitory activity of compounds on human hepatoma cells(HepG2)was determined by the MTT assay,and the anti-oxidant activity was evaluated by ABTS and DPPH methods.RESULTS Twenty-two compounds were isolated and identified as pentacosane(1),kadpolysperin J(2),abiesanolide E(3),manwuweizic acid(4),longipedlactone J(5),7β,15β-dihydroxynigranoic acid(6),xuetongsu(7),phlegmanol K(8),12-angeloyl wuweizidilactone I(9),wuweizidilactone I(10),xuetongdilactone D(11),propindilactone R(12),N-(2-phenylethyl)cinnamamide(13),(-)-(6αS,7R)-7-hydroxyactinodaphnine(14),actinodaphnine(15),cassythicine(16),(+)-3,11,12-trihydroxycalamenene(17),8,9-dihydro-8,9-dihydroxymegastigmatrienone(18),(±)-trans-3-methylidenecyclopropane-1,2-dicarboxylic acid(19),2,4-di-tert-butylphenol(20),palmitic acid(21),and β-sitosterol(22).The IC50 values of compounds 5,14 and 15 against HepG2 cells were(5.23±0.93),(25.84±2.50)and(25.69±2.87)μmol/L,respectively,among which compound 5 exhibited superior activity to the positive controls sunitinib and doxorubicin hydrochloride.IC50 values of compound 11 on ABTS and DPPH radicals were(36.3±1.26)and(72.0±5.81)μmol/L,respectively.CONCLUSION Compounds 2-6 and 8-12 are isolated from I.aromatica for the first time.Compound 5 has significant inhibitory activity against HepG2 cells,and 11 exhibits good in vitro anti-oxidant activity.