基于HPLC指纹图谱、化学计量学和含量测定评价不同商品规格桑椹质量OA
Quality evaluation of Mori Fructus with different commercial specifications based on HPLC fingerprints,chemometrics and content determination
目的 评价黑统、黑选、白统、青统桑椹质量.方法 建立 HPLC 指纹图谱,进行层次聚类分析、主成分分析和正交偏最小二乘判别分析,测定总多酚、总多糖、绿原酸、矢车菊素-3-葡萄糖苷、芦丁含量.结果 121 批药材指纹图谱中有 9 个共有峰,相似度 0.559～0.995,指认出绿原酸、矢车菊素-3-葡萄糖苷和芦丁.各批样品聚为 3 类,3个主成分累积方差贡献率为 84.7％.黑统、黑选药材中各成分含量较高,特征相似,质量较优;青统药材中绿原酸含量较高,多酚、多糖含量最低;白统药材中多糖含量较高,绿原酸、芦丁含量最低.结论 该方法稳定可靠,重复性良好,可用于不同商品规格桑椹的质量控制.
AIM To evaluate the quality of Mori Fructus with Heitong,Heixuan,Baitong and Qingtong.METHODS HPLCfingerprints were established,after which hierarchical cluster analysis,principal component analysis and orthogonal partial least squares discriminant analysis were performed,the contents of total polyphenols,total polysaccharides,chlorogenic acid,cyanidin-3-glucoside and rutin were determined.RESULTS Nine common peaks were identified in HPLC fingerprints of 121 batches,with chlorogenic acid,cyanidin-3-glucoside and rutin authenticated.There were 9 common peaks in the fingerprints for 121 batches of medicinal materials with the similarities of 0.559-0.995,chlorogenic acid,cyanidin-3-glucoside and rutin were identified.Various batches of samples were clustered into 3 types,3 principal constituents demonstrated the accumulative variance contribution rate of 84.7％.The contents of various constituents were high in medicinal materials with Heitong and Heixuan,with similar characteristics and good quality;the content of chlorogenic acid in medicinal material with Qingtong was high,along with the lowest contents of polyphenols and polysaccharides;the content of polysaccharide in medicinal material with Baitong was high,along with the lowest contents of chlorogenic acid and rutin.CONCLUSION This stable,reliable and repeatable method can be used for the quality control of Mori Fructus with different commercial specifications.
万美霞;李庆;王曙;肖雨蝶;冯莉雅;闫捷;张龙梅
陇东学院医学院,甘肃 庆阳 745000||四川大学华西药学院,四川 成都 610000四川大学华西第二医院,四川 成都 610000四川大学华西药学院,四川 成都 610000四川大学华西药学院,四川 成都 610000陇东学院医学院,甘肃 庆阳 745000陇东学院医学院,甘肃 庆阳 745000陇东学院医学院,甘肃 庆阳 745000
医药卫生
桑椹商品规格质量评价HPLC指纹图谱层次聚类分析主成分分析正交偏最小二乘法判别分析含量测定
Mori Fructuscommercial specificationsquality evaluationHPLC fingerprintshierarchical cluster analysisprincipal component analysisorthogonal partial least squares discriminant analysiscontent determination
《中成药》 2026 (9)
2860-2868,9
四川省科技计划项目-重大研发项目(2020YFS0031)陇东学院博士基金项目(XYBYZK2310)
评论