肺源性急性呼吸窘迫综合征"气"损评估的理论和大动物模型探索OA

Objective Acute respiratory distress syndrome(ARDS)is associated with high mortality,and specific therapies remain lacking in clinical practice.This study aimed to construct a clinically relevant large-animal model of pulmonary ARDS,preliminarily establish a multidimensional objective indicator system reflecting impairment of Qi-related functions,and initially explore the intervention effects of an integrative Chinese-Western medicine therapeutic strategy based on the double tonification of tangible and intangible Qi derived from the theory of state differentiation and treatment.Methods At the theoretical level,with"Impairment of Qi-Dysfunction of Qi transformation-Disorder of Qi movement"as the core pathomechanistic chain of ARDS,this study systematically analyzed the potential associations between modern monitoring parameters,including extravascular lung water index(EVLWI),pulmonary vascular permeability index(PVPI),arterial partial pressure of oxygen(PaO2),lactate(Lac),pulse contour cardiac index(PCCI),and static lung compliance(Cst),and the functional state of Qi in traditional Chinese medicine,thereby constructing a dynamic monitoring and analytical framework.At the experimental level,pigs,whose respiratory and circulatory physiology closely resembles that of humans,were used to establish a lipopolysaccharide-induced large-animal model of pulmonary ARDS,followed by a small-sample exploratory study.Animals were assigned to a control group,a model group,a Western medicine group receiving standard supportive treatment,and an integrative Chinese-Western medicine group receiving Western supportive treatment combined with Kaibi Bufei Tang.The above multidimensional parameters and inflammatory cytokines were dynamically monitored,and histopathological analysis of lung tissue was performed at the experimental endpoint.Results A stable porcine model of pulmonary ARDS was successfully established.Typical multidimensional pathophysiological changes of ARDS were observed in this model,and continuous dynamic monitoring of Qi-related functional indicators was achieved.The findings also suggested that Kaibi Bufei Tang combined with Western supportive treatment may have potential therapeutic effects in this model.Conclusion This study preliminarily established an analytical framework linking the functional state of Qi in traditional Chinese medicine with modern critical care monitoring technologies and successfully developed a porcine model of pulmonary ARDS suitable for investigating this framework.This model can be used for dynamic observation of impairment in Qi-related functions and provides a new methodological platform and exploratory basis for mechanistic studies of integrative Chinese-Western medicine strategies such as the double tonification of tangible and intangible Qi.