青附宣肺汤对博来霉素诱导大鼠肺纤维化的干预作用及机制OA

Aim To evaluate the anti-pulmonary fi-brosis effect of Qingfu Xuanfei Tang(QFXFT)and ex-plore its mechanism in a bleomycin-induced rat model.Methods First,to establish a stable model for pharmacodynamic study,SD rats were divided into a control group,a single intratracheal aerosolized bleo-mycin group(4.5 mg·kg-1),and a triple intratracheal aerosolized bleomycin group(1.5 mg·kg-1 per time,once a week).Model stability and fibrosis degree were evaluated by lung histopathology and hydroxyproline(HYP)content.Then,the superior modeling method was adopted.Successfully modeled rats were randomly divided into a model group,a prednisone group(5 mg·kg-1),and QFXFT groups(1,2 g·kg-1),receiving continuous intervention for 28 days.The pathological changes were assessed by HE and Masson staining.The protein expression levels of α-SMA,Col-I,and Col-Ⅲ in lung tissue were detected by Western blot and immunohistochemistry.Results Model compari-son showed that the single administration group had significantly higher alveolar inflammation scores,fi-brosis degree,and HYP content than the triple admin-istration group(all P<0.01).Pharmacodynamic ex-periments showed that,compared with the model group,both 2 g·kg-1 QFXFT group and prednisone sig-nificantly alleviated alveolitis and pulmonary fibrosis pathological damage,and significantly down-regulated the protein expression levels of α-SMA,Col-I,and Col-Ⅲ in lung tissue(P<0.01).Conclusions In an optimized bleomycin aerosol-induced model,QFXFT can significantly inhibit the progression of pulmonary fibrosis,and its mechanism may be related to the inhi-bition of fibroblast activation and extracellular matrix deposition.