天麻素通过调控PI3K信号通路并降低活性氧水平减轻6-羟基多巴胺诱导的PC12细胞凋亡OA
Gastrodin attenuates 6-hydroxydopamine-induced apoptosis in PC12 cells by modulating the PI3K signaling pathway and reducing reactive oxygen species levels
目的:探讨天麻及其主要活性成分天麻素对帕金森病的潜在治疗机制,重点分析其在细胞凋亡、氧化应激及磷脂酰肌醇-3-激酶(PI3K)/RAC 丝氨酸/苏氨酸蛋白激酶(Akt)/丝氨酸/苏氨酸蛋白激酶 mTOR(mTOR)信号通路中的作用.方法:采用 6-羟基多巴胺(6-OHDA)诱导的 PC12细胞损伤模型,通过体外实验评估天麻素对细胞凋亡、细胞内活性氧水平及 PI3K/Akt/mTOR 信号通路相关蛋白表达的影响.结果:实验结果表明,天麻素可显著改善 6-OHDA 诱导的PC12细胞凋亡,并对 PI3K 信号通路活性产生调控作用.此外,天麻素能够有效降低细胞内活性氧水平,该作用不依赖于 PI3K 信号通路的调控.结论:天麻素可能通过降低细胞内活性氧水平并调控 PI3K/Akt/mTOR 信号通路的活性,协同发挥神经保护作用,从而减轻 6-OHDA 诱导的细胞凋亡,为其在帕金森病防治中的应用提供实验依据.
AIM:To investigate the potential ther-apeutic mechanisms of Gastrodia elata and its main active component gastrodin(GSD)in Parkinson's disease(PD),focusing on apoptosis,oxidative stress,and the PI3K/Akt/mTOR signaling pathway.METHODS:A 6-hydroxydopamine(6-OHDA)-in-duced PC12 cell damage model was used to evalu-ate the effects of GSD on cell apoptosis,intracellu-lar reactive oxygen species(ROS)levels,and the ex-pression of PI3K/Akt/mTOR signaling pathway-relat-ed proteins in vitro.RESULTS:GSD significantly at-tenuated 6-OHDA-induced apoptosis in PC12 cells and modulated the activity of the PI3K signaling pathway.In addition,GSD effectively reduced intra-cellular ROS levels,and this effect was independent of PI3K pathway regulation.CONCLUSION:Gas-trodin may exert neuroprotective effects by reduc-ing intracellular ROS levels and modulating the PI3K/Akt/mTOR signaling pathway,thereby alleviat-ing 6-OHDA-induced apoptosis in PC12 cells.These findings provide experimental evidence supporting the potential application of GSD in PD therapy.
张鑫宇;温绍文;赵斐;吴丽丽;张炅璐;房泽海;刘红杰
暨南大学中医学院,广州 510632,广东暨南大学中医学院,广州 510632,广东淄博职业技术大学护理学院,淄博 255300,山东暨南大学中医学院,广州 510632,广东暨南大学中医学院,广州 510632,广东淄博职业技术大学护理学院,淄博 255300,山东暨南大学中医学院,广州 510632,广东
医药卫生
天麻天麻素PI3K/Akt/mTOR信号通路细胞凋亡
Gastrodia elata BlumeGastrodinPI3K/Akt/mTOR signaling pathwaycell apoptosis
《中国临床药理学与治疗学》 2026 (9)
1153-1161,9
国家自然科学基金面上项目(82074306)第五批全国中医临床优秀人才研修项目(国中医药人教函[2022]239号)
评论