肺气虚寒证型肺纤维化小鼠模型的构建及评价OA

AIM:To establish and evaluate a mouse model of pulmonary fibrosis with the lung Qi deficiency and cold pattern.METHODS:Forty C57BL/6J mice were randomly assigned to four groups:control,bleomycin(BLM),lung Qi deficiency and cold pattern(LC),and LC+BLM groups(n=10).The model was established using"cold water in-take+cold environment exposure+forced swimming in ice water"combined with intranasal BLM administration.Clinical signs were observed in each group,and lung function and behavioral changes were assessed.Lung tissues were collected to compare pathological changes,hydroxyproline content,inflammatory factor levels,and energy metabolism enzyme ac-tivities among groups.RESULTS:Compared with the control group,the LC and LC+BLM groups had lower rectal tem-peratures(P<0.01)and reduced energy metabolism(P<0.01).Their clinical signs(R≥3)and body-weight changes(P<0.01)were consistent with the lung Qi deficiency and cold pattern.In the BLM and LC+BLM groups,the lung coefficient increased(P<0.01),lung function declined(P<0.05),and hydroxyproline content and inflammatory cytokine levels in-creased significantly(P<0.01).Marked inflammatory infiltration and fibrosis were observed in lung tissue(P<0.01),in-dicating successful induction of pulmonary fibrosis.CONCLUSION:"Cold water intake+cold environment exposure+swimming to exhaustion in ice water"combined with intranasal BLM administration can establish a C57BL/6J mouse model of pulmonary fibrosis with the lung Qi deficiency and cold pattern.This simple and effective method closely mimics natural disease progression.