某院侵袭性念珠菌感染分离株的药物敏感性及微卫星基因分型OA

Objective To investigate the species composition,antifungal resistance characteristics,and genotyping of Candida isolates from invasive candidiasis at a hospital,thereby providing evidence for the rational clinical use of antifungal agents and source tracing of cases.Methods Candida isolates from invasive candidiasis collected at Hebei Yanda Hospital between June 2019 and December 2025 were analyzed for species distribution and antifungal resistance.Species identification was confirmed using the VITEK MS mass spectrometry system.In vitro antifungal susceptibility testing against nine antifungal agents was performed with fungal susceptibility testing panels.Microsatellite markers were used for genotyping of the isolates.Results A total of 132 Candida isolates from invasive candidiasis were recovered.Candida albicans was the most common species,with 53 isolates(40.15％),followed by Candida parapsilosis species complex with 33(25.00％),Candida tropicalis with 22(16.67％),Candida glabrata with 18(13.64％),Candida auris with 2(1.51％),and other Candida species with 4(3.03％).The correct identification rate after confirmatory identification was 85.61％(113/132).C.albicans showed low resistance rates(＜8％)to all nine antifungal agents,whereas the resistance rates of C.tropicalis to both fluconazole and voriconazole were 54.5％.Microsatellite genotyping revealed that Candida isolates recovered from different body sites of the same patient shared identical genotypes.Conclusion C.albicans remained the predomi-nant species among Candida isolates from invasive candidiasis and showed high susceptibility to most antifungal agents.C.tropicalis showed cross-resistance to azoles.Microsatellite genotyping indicated genotypic polymorphism among C.albicans,C.parapsilosis,and C.tropicalis isolates,and the genotypes of isolates causing invasive infections were associated with those of colonizing isolates.