罗布麻叶总黄酮提取工艺的优化及抗氧化、降糖活性的研究OA

To optimize the extraction process for total flavonoids from Apocynum venetum leaves and evaluate their antioxidant capacity and hypoglycemic activity in vitro.The optimal process conditions were ethanol volume fraction of 71%,extraction temperature 70℃,solid-to-liquid ratio 1∶14 g/mL based on the orthogonal test combined with the central composite design,the verified extraction rate of which reached 14.87%.In vitro antioxidant experiments showed that the half maximal inhibitory concentration(IC50)values of total flavonoids from Apocynum venetum leaves against 2,2-diphenyl-1-picrylhydrazyl(DPPH)and Hydroxyl radical(·OH)were 4.15 and 13.24 μg/mL,respectively,with scavenging capacities 0.67 and 0.73 times that of vitamin C(Vc).In terms of hypoglycemic activity,the IC50 values of total flavonoids from Apocynum venetum leaves against for α-glucosidase and α-amylase were 0.72 and 0.85 mg/mL respectively,which were lower than those of acarbose(IC50 values of 0.18 and 0.20 mg/mL,respectively).The results indicated that the optimized process was stable and feasible,with a high extraction rate of total flavonoids from Apocynum venetum leaves and good antioxidant capacity and hypoglycemic activity.