基于SNP标记的泰和乌鸡群体结构分析及关键基因挖掘OA

In this study,Taihe silky fowl were genotyped using the Jingxin No.1 SNP array,and publicly available genome-wide data from six indigenous chicken breeds in Jiangxi(Xiushui yellow-feathered black-bone chicken,Chongren partridge chicken,Kangle chicken,Dongxiang blue-shelled chicken,Guangfeng white-eared yellow chicken,and Anyi gray chicken)were integrated for population structure and runs of homozygosity(ROH)analyses.Taihe silky fowl exhibited a relatively distinct genetic cluster and ancestry component.A total of 1 257 ROH segments were identified in 50 individuals,of which 83.45%were 1-5 Mb in length.The genomic inbreeding coefficient estimated from ROH(FROH)was 0.045-0.135,with a mean value of 0.073±0.018.Twelve candidate ROH islands containing 98 protein-coding genes were identified.These genes were significantly enriched in signaling pathways including Hippo,Wnt,and PI3K-Akt.Candidate genes included ITGB2,LGR4,COL6A1,LAP3,NTF3,and WNT6,which are involved in signal regulation,cell adhesion and migration,membrane and nuclear components,and protein binding.In conclusion,Taihe silky fowl possesses a relatively distinct genetic background.Although the population showed no widespread evidence of recent intensive inbreeding,the degree of genomic inbreeding varied among individuals.