异色瓢虫对日本松干蚧的捕食能力OA

[Aim]The Japanese pine bast scale,Matsucoccus matsumurae,is an important invasive pest in pine forests in China.To explore the biological control strategies of M.matsumurae,this study systematically evaluated the predatory capacity and biological control potential of the multicolored Asian lady beetle,Harmonia axyridis,against the 2nd instar nymphs,adult females and oocysts of M.matsumurae.[Methods]Under laboratory conditions,the handing time and predation amount of adults and the 2nd-3rd instar larvae of H.axyridis on the 2nd instar nymphs,adult females and oocysts of M.matsumurae were measured.The Holling disc equation was used to model the predatory functional responses of adults and the 2nd-3rd instar larvae of H.axyridis to M.matsumurae,and the instantaneous attack rate,theoretical maximum daily predation amount and predation capacity were calculated.The predatory preference of H.axyridis to different developmental stages of M.matsumurae was analyzed using the Cain index,and the interference effects of the density of H.axyridis on its predation capacity on the 2nd instar nymphs of M.matsumurae was assessed with the Hassell-Varley model.[Results]The predatory efficiency of the 2nd-3rd instar larvae of H.axyridis on the 2nd instar nymphs of M.matsumurae(theoretical maximum daily predation amount:169.5 individuals;predatory capacity:164.2 individuals/d)was higher than that of H.axyridis adults on the female adults of M.matsumurae(theoretical maximum daily predation amount:41.8 individuals;predatory capacity:44.2 individuals/d).The type of predatory functional response varied with the predator-prey combination:The predation of H.axyridis adults on the 2nd instar nymphs of M.matsumurae and that of the 2nd-3rd instar larvae of H.axyridis on the adult females of M.matsumurae fitted the Holling type Ⅲ model,whereas the predation of the 2nd-3rd instar larvae of H.axyridis on the 2nd instar nymphs of M.matsumurae and that of H.axyridis adults on the adult females of M.matsumurae fitted the Holling type Ⅱmodel.Predatory preference analysis revealed that H.axyridis significantly preferred adult females of M.matsumurae over the 2nd instar nymphs or oocysts in all combinations,with all Cain indices being greater than 1.The intraspecific interference response showed that the predation rates of adults and the 2nd-3rd instar larvae of H.axyridis on the 2nd instar nymphs of M.matsumurae exhibited a decreasing trend with the predator density increasing,the interference coefficient(m)was greater than 1,and the intensity of scramble competition exceeded 0.8 at the density of 5 predators per container.[Conclusion]H.axyridis exhibits strong predation potential against M.matsumurae.For field applications,it is recommended to prioritize the release of larval H.axyridis,which exhibits a Holling type Ⅱ functional response,during the peak occurrence period of the 2nd instar nymphs of M.matsumurae,while rationally controlling the release density to enhance practical control efficacy.This study provides a theoretical foundation for the scientific utilization of H.axyridis in the integrated management of M.matsumurae.