产香酵母XW2-1的分离鉴定及其在烟用香料制备中的应用OA

To utilize waste tobacco for preparing tobacco flavoring agents,aroma-producing strains were screened and isolated from tobacco leaves.The isolated strain was identified based on morphological characteristics combined with ITS sequence analysis.The optimal growth conditions for the strain were determined using single-factor experiments,after which it was inoculated into waste tobacco for solid-state fermentation.Volatile aroma components in the fermentation product were analyzed by gas chromatography-mass spectrometry(GC-MS).The fermentation product was then processed into a tobacco extract for cigarette flavoring evaluation.The results showed that a highly effective aroma-producing strain,designated XW2-1,was isolated from tobacco leaves in Xuanwei,Yunnan Province,and identified as Clavispora lusitaniae.The logarithmic growth phase of strain XW2-1 occurred between 4 and 16 h.Its optimal growth conditions were determined as follows:temperature 28 ℃,pH 6,and shaking speed 200 r/min.Compared to the control,fermentation with strain XW2-1 significantly increased both the variety and quantity of volatile aroma components,producing various flavor compounds such as benzyl alcohol,4,7,9-megastigmatrien-3-one,linalool,and methyl linolenate.The extract prepared from the fermentation broth of strain XW2-1 significantly improved the sensory quality of cigarettes.At a dosage of 1‰,the sensory score of the cigarettes reached the highest,with prominent floral notes,increased aroma intensity,enhanced sweetness,and a clean aftertaste.