精子相关抗原9、癌胚抗原、糖类抗原19-9联合检测对结直肠癌诊断的相关性研究OA

Objective To investigate the diagnostic value of sperm-associated antigen 9(SPAG9),carcinoembryonic antigen(CEA)and carbohydrate antigen 19-9(CA19-9)in colorectal cancer(CRC)patients,colorectal benign lesions patients and healthy subjects.Methods A total of 88 CRC patients admitted to Hongqi Hospital Affiliated to Mudanjiang Medical Uni-versity from September 2022 to September 2025 were selected as the case group.A total of 88 patients with benign colorectal lesions and 88 healthy individuals undergoing physical examinations were selected as the benign disease group and the healthy control group,respectively.The expression levels of SPAG9 in the serum of the three groups were detected by enzyme-linked immunosorbent assay,the expression levels of CEA and CA19-9 were determined by chemilumines-cence method,the relationships between the expression levels of SPAG9,CEA and CA19-9 in the case group and the depth of tumor invasion,pathological stage and lymph node metastasis were analyzed.The receiver operating characteristic(ROC)curve was drawn to evaluate the diagnostic value of SPAG9,CEA and CA19-9 alone and in combination for CRC.Results The expression levels of SPAG9,CEA and CA19-9 in the case group were higher than those in the benign disease group and the healthy control group,and the differences were statistically significant(P<0.05).There were statistically significant differences in the levels of CEA and CA19-9 among patients with different tumor invasion depths(P<0.05).Among the T3 and T4 groups,the expression levels of CEA and CA19-9 were higher than those in the T1 group,and the differences were statistically significant(P<0.05).There were statistically significant differences in the levels of CEA and CA19-9 among patients with different TNM stages(P<0.05).Among the Ⅱ,Ⅲ and Ⅳ stages,the expression levels of CEA and CA19-9 were higher than those in the Ⅰ stage,and the differences were statistically significant(P<0.05).The expression levels of SPAG9 and CA19-9 in the group with lymph node metastasis were higher than those in the group without lymph node metastasis,and the differences were statistically significant(P<0.05).Conclusion The combined detection of SPAG9,CEA and CA19-9 in CRC has a better clinical diagnostic efficacy than their individual detections,moreover,the expression level of SPAG9 is related to lymph node metastasis in CRC patients.