猫须草水提物的急性毒性及镇痛抗炎活性OA

Objective To investigate the acute toxicity and analgesic and anti-inflammatory effects of the aqueous extract of Clerodendranthus Spicatus(Thunb.)from Xishuangbanna,and to provide a basis for clinical application.Methods The acute toxicity test was conducted on KM mice using the maximum dose(three intragastric administrations per day,with a cumulative dose of 42 g/kg of the crude drug).The test was observed for 14 days,and the changes in mice body weight and organ morphology were recorded.The inhibitory effect of the aqueous extract of Clerodendranthus Spicatus(Thunb.)on the writhing response of mice was observed using the acetic acid writhing method.The anti-inflammatory effects of the drug were evaluated using two methods:xylene-induced ear swelling and egg white-induced foot swelling.Results All mice survived in the acute toxicity test.There was no statistically significant difference in the weight difference between the aqueous extract of Clerodendranthus Spicatus(Thunb.)group and the blank group(P>0.05),and no abnormalities were observed in the main organs.In the analgesic test,the number of writhing times of mice in the high-dose group,medium-dose group,and low-dose group of the aqueous extract of Clerodendranthus Spicatus(Thunb.)were lower than those of the model group,and the number of writhing times of mice in the high-dose group of the aqueous extract of Clerodendranthus Spicatus(Thunb.)was lower than that of the Ibuprofen group,with statistically significant differences(P<0.05).The degree of ear swelling in the high-dose group,medium-dose group,and low-dose group of aqueous extract of the Clerodendranthus Spicatus(Thunb.)were lower than those of the model group,and the degree of ear swelling in the high-dose group of the aqueous extract of Clerodendranthus Spicatus(Thunb.)was lower than that of the Ibuprofen group,with statistically significant differences(P<0.05).The degree of foot swelling at 1,2,4,6,and 24 hours in the high-dose group,medium-dose group,and low-dose group of the aqueous extract of Clerodendranthus Spicatus(Thunb.)were lower than those of the model group,and the degree of foot swelling at 2,4,and 6 hours in the high-dose group,medium-dose group of the aqueous extract of Clerodendranthus Spicatus(Thunb.)were lower than those of the Ibuprofen group,with statistically significant differences(P<0.05).Conclusion The aqueous extract of Clerodendranthus Spicatus(Thunb.)is safe at the experimental dose and has no obvious acute toxicity.It also has significant analgesic and anti-inflammatory pharmacological activities.The analgesic and anti-inflammatory effects of the aqueous extract of Clerodendranthus Spicatus(Thunb.)show a dose-dependent manner.