鱼类耳石荧光染色标记技术研究进展OA

Global fishery resources have long been subjected to considerable fishing pressure,and stock enhance-ment has become a vital measure for the restoration and management of fishery resources.Nevertheless,the effec-tiveness of stock enhancement is influenced by multiple factors,such as environmental conditions,release strategies,and individual differences,making it difficult to accurately quantify its actual contribution solely based on the re-lease scale.Hence,reliable techniques are urgently required for individual tracking and source attribution.Fluores-cent otolith marking can achieve batch marking of small individuals,such as larvae and juveniles,as well as in large-scale groups and can technically support release attribution,survival estimation,and population structure evaluation.This paper focuses on fluorescent otolith marking technology for fish;reviews the basic principles of otolith biomineralization and fluorescent marking;compares the marking efficiency,long-term retention,toxicity risks,and application scenarios of commonly used markers,including calcein,alizarin red S,alizarin complexone,and tetracycline compounds;and summarizes technical routes,such as immersion,injection,and double or multiple marking.On this basis,a safety evaluation framework for fluorescent otolith marking is further developed,with a focus on acute damage,sublethal effects,growth and behavioral effects,potential biases in stock enhancement as-sessment,marker residues,and food safety risks.The paper also proposes prerelease marking quality control,re-lease-process documentation,post-recapture sample interpretation,marking-rate correction,and technical standard-ization measures to provide a reference for the scientific evaluation of stock enhancement and the standardized ap-plication of fluorescent otolith marking technology.