不同丛枝菌根真菌对烟苗根系抗氧化酶活性及真菌群落多样性的影响OA
Effects of Different Arbuscular Mycorrhizal Fungi(AMF)on Antioxidant Enzyme Activity and Fungal Community Diversity of Tobacco Seedling Root System
[目的]探明接种不同丛枝菌根真菌(Arbuscular Mycorrhizal Fungi,AMF)对烤烟成苗期根系生长发育、抗氧化酶活性及真菌群落多样性的影响,为苗期接种 AMF提升烟苗质量提供参考.[方法]采用漂浮育苗方式,以接种等量 3种 AMF复合灭活真菌为对照(CK),研究接种不同 AMF处理[Fm:摩西斗管囊霉(Funneliformis mosseae)、Ri:根内根孢囊霉(Rhizophagus intraradices)、Ce:幼套近明球囊霉(Claroi-deoglomus etunicatum)、Fm_Ri_Ce:等量复配上述 3种菌剂]对烤烟成苗期根系生长发育、抗氧化酶活性及真菌群落多样性的影响.[结果]4种 AMF处理均可与烟苗根系建立稳定的共生关系.与 CK比,接种 AMF处理烟苗的地上部干重(38.9%～79.6%)、根部干重(78.6%～96.4%)、过氧化氢酶(23.0%～36.1%)、过氧化物酶(55.9%～117.9%)、超氧化物歧化酶(53.3%～124.3%)活性均显著提高,苯丙氨酸解氨酶活性提高17.4%～169.8%,丙二醛含量(31.5%～50.0%)显著降低;接种单一 AMF烟苗的总根长、根系投影面积、根系表面积、根系体积和总根尖数均显著增加.利用高通量测序技术对 15个根系样本进行检测,共获得有效基因序列 5 667 671条,平均序列长度 224 bp,共检出 159个 OTUs.其中,Ri、Ce的 OTUs总数(92个、81个)和特有 OTUs数量(19个、17个)均较多.真菌群落多样性分析表明,Ri和 Ce的烟苗根系真菌群落结构与其他处理存在显著差异,其真菌群落丰富度、多样性和均匀度均显著高于其他处理.[结论]单独接种 AMF可有效促进烟苗根系生长发育;在提高抗逆性及增强真菌群落多样性方面,以单独接种根内根孢囊霉、幼套近明球囊霉的效果较为显著.
[Objective]The effects of different arbuscular mycorrhizal fungi(AMF)on growing development,antioxidant enzyme activity and fungal community diversity of tobacco seedling root system at seedling stage were investigated to provide a reference for promoting quality of tobacco seedlings by inoculating AMF.[Method]The effects of 5 treatments including CK(without inoculating AMF),Fm(inoculating Funneliformis mosseae),Ri(inoculating Rhizophagus intraradices),Ce(inoculating Claroideoglomus etunicatum)and Fm_Ri_Ce(inoculating equivalent F.mosseae,R.intraradices and C.etunicatum)on growing development,antioxidant enzyme activity and fungal community diversity of tobacco seedling root system at seedling stage were studied by using the floating seedling cultivation method.[Result]Four AMF treatments could establish a stable symbiotic relationship with the root system of tobacco seedlings.The above-ground dry weight,root dry weight,CAT activity,POD activity,SOD activity and PAL activity of tobacco seedling roots inoculated with AMF increased by 38.9%-79.6%,78.6%-96.4%,23.0%-36.1%,55.9%-117.9%,53.3%-124.3%and 17.4%-169.8%compared with those of CK,respectively.The MDA content in tobacco seedling roots inoculated with AMF reduced by 31.5%-50.0%compared with that of CK.The total root length,root projection area,root surface area,root volume and total root tips of tobacco seedling roots from Fm,Ri and Ce treatments increased significantly.A total of 5 667 671 effective gene sequences with an average sequence length of 224 bp were detected from 15 samples by high-throughput sequencing technology.A total of 159 OTUs were detected.92 and 81 OTUs were detected in Ri and Ce treatments,respectively.19 and 17 unique OTUs were detected in Ri and Ce treatments,respectively.The analysis of fungal community diversity showed that the fungal community structure of tobacco seedling roots in Ri and Ce treatments was significantly different from those in other treatments,and the richness,diversity and evenness of the fungal communities in Ri and Ce treatments were significantly higher than those in the other treatments.[Conclusion]Inoculating single AMF can promote growing development of tobacco seedling roots effectively.Inoculating single R.intraradices or C.etunicatumi has the significant effects on improvement of stress resistance and increase of fungal community diversity for tobacco seedling roots.
张恒;褚玉麟;王明鑫;许跃奇;王晓强;阎海涛;张富生;阴广宇;常栋
河南省烟草公司 平顶山市公司,河南 平顶山 467000河南省烟草公司 平顶山市公司,河南 平顶山 467000河南省烟草公司 平顶山市公司,河南 平顶山 467000河南省烟草公司 平顶山市公司,河南 平顶山 467000河南省烟草公司 平顶山市公司,河南 平顶山 467000河南省烟草公司 平顶山市公司,河南 平顶山 467000河南省烟草公司 平顶山市公司,河南 平顶山 467000河南省烟草公司 平顶山市公司,河南 平顶山 467000河南省烟草公司 平顶山市公司,河南 平顶山 467000
农业科技
丛枝菌根真菌烟苗生长发育根系抗氧化酶活性真菌群落多样性
arbuscular mycorrhizal fungi(AMF)tobacco seedlinggrowing developmentroot systemantioxidant enzyme activityfungal community diversity
《贵州农业科学》 2026 (10)
81-89,9
河南省烟草公司青年人才项目"基于丛枝菌根真菌提升烤烟抗性和品质的应用技术研究"(202503003)
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