绿肥配施化肥对土壤微生物及钩藤产质量与氮肥利用率的影响OA

[Objective]The effects of green manure combined with chemical fertilizer on structure of soil microbial communities,and yield,quality and nitrogen use efficiency of Uncaria rhynchophylla were investigated to provide technical support for green and efficient production of U.rhynchophylla.[Method]The effects of 6 treatments including S1(no fertilization),S2(single applying chemical fertilizer),S3(chemical fertilizer combined with green manure),S4(green manure replacing 15%of chemical fertilizer),S5(green manure replacing 30%of chemical fertilizer)and S6(green manure replacing 45%of chemical fertilizer)on microbial community structure at the genus level,biomass of different parts of U.rhynchophylla,and quality,nitrogen absorption and nitrogen use efficiency of U.rhynchophylla were investigated.[Result]The relative abundance of unclassified_p_Acidobacteria in soil samples of different treatments was the highest,and the unclassified genus was within the Acidobacteria phylum.Compared with S1,the relative abundance of unclassified_p_Acidobacteria in S2 decreased by 14.98%but the relative abundance in S6 increased by 19.14%.The fresh and dry weight of twigs,stems and leaves of U.rhynchophylla plants from all treatments increased to varying degrees,and the highest value was observed in S5.The fresh and dry twig weight increased by 64.18%and 56.76%compared with that of S1.The content of rhynchophylline,isorhynchophylline,corynoxeine,isocorynoxeine and total alkaloids in S5 significantly increased by 23.66%,27.67%,156.02%,126.30%and 18.34%compared with those in S1,respectively.Compared with S2,total nitrogen absorption and nitrogen use efficiency of S4 and S5 plants were significantly improved.Total nitrogen absorption,nitrogen apparent use efficiency,nitrogen physiological use efficiency,partial productivity and agronomic efficiency of S5 plants increased by 33.33%,253.06%,43.33%,96.54%and 4.06 times compared with those of S2,respectively.[Conclusion]The fertilization pattern of green manure combined with an appropriate amount of chemical fertilizer can effectively regulate soil microbial community structure and alleviate the inhibitory effect of chemical fertilizer on microbial relative abundance.The relative abundance of soil microbial communities shows an increasing trend with the increase of nitrogen replacement proportion based on adding green manure.The fertilization pattern of green manure combined with chemical fertilizer can significantly improve biomass of different parts,content of medicinal components and total alkaloids,total nitrogen absorption and nitrogen use efficiency in U.rhynchophylla.Overall,the fertilization pattern of green manure replacing 30%of nitrogen fertilizer can produce the best results in the production of U.rhynchophylla.