扶正化瘀胶囊、一贯煎和茵陈蒿汤对刀豆蛋白A诱导大鼠肝纤维化的作用比较OA

Objective:To investigate the mechanisms of action of Fuzheng Huayu capsule,Yiguanjian,and Yinchenhao decoction on concanavalin A(Con A)-induced hepatic fibrosis(HF)in rats.Methods:A total of 53 Wistar rats were randomly divided into a normal control group(n=10)and a model induction group.Except for those in the normal control group,all rats received tail vein injection of Con A to establish the HF animal model.After successful model establishment,the model rats were randomly divided into the model group,Fuzheng Huayu capsule group(hereinafter referred to as the Fuzheng Huayu group),Yiguanjian group,and Yinchenhao decoction group.The normal and model groups received distilled water via intragastric gavage,whereas the Yiguanjian,Yinchenhao decoction,and Fuzheng Huayu groups were treated with corresponding drug solutions at doses of 7.58 g·kg⁻¹,5.13 g·kg⁻¹,and 0.525 g·kg⁻¹,respectively,for four consecutive weeks.Body weight and liver index were recorded throughout the modeling and treatment periods.Serum biochemical parameters,including aspartate aminotransferase(AST),alanine transaminase(ALT),hyaluronic acid(HA),laminin(LN),procollagen type Ⅲ(PC Ⅲ),collagen type Ⅳ(C-Ⅳ),α-smooth muscle actin(α-SMA),matrix metalloproteinase-1(MMP-1),and tissue inhibitor of metalloproteinase-1(TIMP-1),together with hepatic hydroxyproline(Hyp)content,were determined for all groups.Liver tissue morphology was observed and compared among all groups.Results:During the modeling period,the body weight gain of rats in the model group was significantly lower than that in the normal control group(P<0.01).During the intervention period,body weight increased in all groups,but there was no statistically significant difference among the groups(P>0.05).No statistically significant difference was observed in liver index among the groups(P>0.05).In comparison with the normal control group,serum levels of AST,ALT,Hyp,HA,LN,PC Ⅲ,C-Ⅳ,α-SMA,and TIMP-1 were significantly increased in the model group(P<0.01),whereas MMP-1 level and the MMP-1/TIMP-1 ratio were significantly decreased(P<0.01).In comparison with the model group,all three treatment groups(Fuzheng Huayu,Yiguanjian,and Yinchenhao decoction)showed significant amelioration of the aforementioned indicators(P<0.05,P<0.01).Liver histopathology showed varying degrees of improvement in all treatment groups.Hepatocytes were arranged in a more orderly manner,with less cellular deformity and necrosis.Some hepatic lobules retained relatively intact architecture,and fibrous tissue hyperplasia was attenuated compared with the model group.Conclusion:Fuzheng Huayu Capsule,Yiguanjian,and Yinchenhao decoction all attenuated liver dysfunction and HF indices in Con A-induced immunological HF rats,possibly through inhibiting hepatic stellate cell activation and restoring the MMP/TIMP balance.