7株菌株促生特性测定以及对箭筈豌豆生长的影响OA

[Objective]To investigate the effects of inoculation with different bacterial strains on the growth of Vicia sativa and identify the strains with the greatest potential to promote plant growth.[Method]Seven strains se-lected from a strain collection were isolated and purified.Their nitrogen-fixing capacity,phosphate-solubilizing ability,and auxin secretion capacity were evaluated using acetylene reduction assay,molybdenum blue colorimetric method,and liquid chromatography,respectively.The activities of nitrogenase,phosphate solubilization,and auxin se-cretion were further determined using plate contrast method.The seven strains were then formulated as rhizosphere inoculants and applied to V.sativa to evaluate their effects on plant growth.[Results]All seven strains exhibited ni-trogen-fixing,phosphorus solubilizing,and auxin secretion.Strain GAU84 showed the highest nitrogen-fixing capacity and auxin secretion,with values of 62.42 nmol C2H4 nmol/ml·h and 76.08 µg/mL,respectively,whereas strain Y1 exhibited the highest phosphate-solubilizing capacity(237.10 mg/L).Compared with the control group,the inocula-tion with strain Y3 resulted in the greatest improvements in plant height,stem diameter,fresh weight,and dry weight,with increases of 25%and 0.04%reported for two of these growth parameters.[Conclusion]Based on the overall evaluation of the measured traits,strains Y1,Y3,and GAU84 were identified as the most promising strains for promoting the growth of V.sativa.