扁蓿豆SUT基因家族鉴定及在干旱寒冷胁迫下的表达分析OA

[Objective]To elucidate the potential functional roles of sucrose transporters(SUTs)in Medicago ruthenica.[Method]The MrSUT gene family was identified through genome-wide analysis and characterized using bioinformatic approaches.The expression profiles of MrSUT genes under drought and cold stress were subsequently analyzed by quantitative real-time PCR(qRT-PCR).[Result]Seven MrSUT genes were identified and phylogeneti-cally classified into three clades(SUT1,SUT2,SUT4).These genes were designated as MrSUT1-1,MrSUT1-2,MrSUT1-3,MrSUT1-4,MrSUT2,MrSUT4-1,and MrSUT4-2.Ten conserved motifs,ranging from 21-50 amino acids in length,were identified,each containing multiple conserved residues.Analysis of the promoter regions re-vealed various cis-acting elements associated with light responsiveness,hormone signaling,abiotic stress responses,and growth regulation.qRT-PCR analysis showed that MrSUTs were expressed in both leaves and roots.Under 15%PEG(drought)and 4℃(cold)treatments,the expression of MrSUT1-4 in leaves was significantly upregulated within 6-12 h,whereas MrSUT1-2 in roots was markedly upregulated under both stress conditions.[Conclusion]MrSUT1-2 may play an important role in the response of M.ruthenica to drought and cold stress.These findings provide a foundation for further functional characterization of the MrSUT gene family in response to abiotic stresses.