解淀粉芽孢杆菌微生物菌剂施用量对兰州百合根区土壤理化性质及微生物结构的影响OA

[Objective]To investigate the effects of a Bacillus amyloliquefaciens-based microbial agent on plant growth,soil nutrient status,and rhizosphere microbial communities of Lanzhou lily.[Method]Four treatments were established:CK(control),T1(6 kg/667 m2 bacteriophage),T2(9 kg/667 m2 bacteriophage),and T3(12 kg/667 m2 bacteriophage),and the microbial structure as analyzed based on both 16S rRna and ITS high-throughput sequenc-ing,respectively.[Result]The Bacillus amyloliquefaciens microbial agent significantly promoted the growth of Lan-zhou lily and significantly increased the root-to-shoot ratio,with(9 kg/667 m²)showing the best overall effect.Com-pared with CK,T2 significantly increased plant height,stem diameter,and the root-to-shoot ratio by 20.41％,19.13％,232.39％,49.22％,respectively,while another measured growth parameter increased by 49.22％.T2 treatment also significantly increased soil organic matter,alkali-hydrolyzable nitrogen,available phosphorus,and avail-able potassium contents.In addition,the microbial agent increased the alpha diversity of both bacterial and fungal com-munities and promoted the enrichment of beneficial genera,including Mortierella and Sphingomonas.[Conclusion]Application of the B.amyloliquefaciens microbial agent improved soil nutrient availability,enhanced rhizosphere mi-crobial diversity and community structure,and promoted the growth of Lanzhou lily.Among the tested treatments,T2(9 kg/667 m²)combined with chemical fertilizer produced the best overall effect and may therefore be an effective application rate for Lanzhou lily production.