叠氮化钠诱导燕麦突变体筛选及叶色突变体表型与光合特性分析OA

[Objective]Provides foundational materials for research on the regulatory mechanisms of oat photo-synthesis,chlorophyll synthesis and degradation,as well as functional genes related to leaf color.[Method]The wild type(WT)was used as the control,and the M2 generation population of oats(Avena sativa)induced by NaN3 was screened for mutants by observing the phenotypes.Photosynthetic characteristics and chlorophyll content of the WT and the leaf color variant mutants were determined by using photosynthesis assay and ethanol extraction,respec-tively.Key agronomic traits were measured in the sizing stage.[Result]A total of eight different types of mutants,in-cluding shrunken leaf,shrunken stem,higher plant variation,and tiller number variation,were isolated from the M2 population,with an average mutation frequency of 0.65%.Further analysis of the white-striped leaf mutant revealed that irregular white stripes appeared on the leaves starting at the seedling stage and extended to leaves across the en-tire plant as development progressed,leading to marked reductions in pigment contents and photosynthetic capacity.Except for chlorophyll b,all pigment contents in the white-striped leaf mutant were significantly lower than those in the WT,with carotenoids exhibiting the largest decline(43.80%).In addition,the net photosynthetic rate,transpira-tion rate,stomatal conductance and intercellular CO2 concentration of the zebra leaf mutant decreased by 68.23%,70.09%,66.85%and 12.61%,respectively,compared with the wild type(P<0.05).The decrease of pigment con-tent and photosynthetic performance inhibited the growth and development of oats.The agronomic traits of the leaf color mutants also showed a downward trend.Among them,the grain number and grain weight of the main panicle de-creased significantly,which were 16.95%and 26.59%,respectively.[Conclusion]The NaN3-induced oat mutant population had a wide range of mutations and a variety of mutation types.The zebra leaf mutant had lower photosyn-thetic characteristics and seed yield components than the wild type.