三种植物提取液对党参根腐病病原菌的抑制效应OA

Taking Allium sativum,Sophora flavescens and Artemisia argyi as experimental materials,the antifungal activities of aqueous extracts from the three plants against two major pathogens causing Codonopsis pilosula root rot,namely Fusarium oxysporum and Fusarium acuminatum,at varied concentrations were determined via the mycelial growth rate method.High-performance liquid chromatography(HPLC)combined with reference standard verification was adopted to quantify the key antifungal constituents,in order to provide references for the exploitation of botanical fungicides and intercropping cultivation between Codonopsis pilosula and the three antifungal plants.The results showed that all three aqueous extracts exerted remarkable inhibitory effects on the two tested pathogens in a concentration-dependent and strain-specific manner.The antifungal activity of garlic extract increased continuously with rising concentration.For S.f lavescens extract,the optimal inhibitory efficacy against F.oxysporum occurred at 0.05 g·mL-1,while the maximum inhibition on F.acuminatum was obtained at 0.1 g·mL-1.As for A.argyi extract,the optimum antifungal concentrations were 0.012 5 g·mL-1 for F.oxysporum and 0.025 g·mL-1 for F.acuminatum,respectively.HPLC analysis confirmed the existence of target antifungal components,and subsequent bioassays with reference standards further verified their antifungal capacities.The peak inhibition rates against F.oxysporum and F.acuminatum reached 69.80% and 75.07%,respectively.