黑木耳液体菌种发酵工艺优化及关键技术参数筛选OA

Taking the'Tai'er No.2'Auricularia auricula strain selected and bred by Tai'an Academy of Agricultural Sciences as the test material,through liquid strain optimization experiments and shake flask strain production process experiments,the nutritional ratio of the liquid strain,the cultivation conditions of shake flasks,as well as the key technical indicators of inoculation and harvest were studied,in order to solve the core problems of unstable strain quality and scarce excellent strains in the domestic Auricularia auricula industry,and to provide a reference for the large-scale application and promotion of this excellent strain.The results showed that the optimal carbon and nitrogen sources for liquid spawn of'Tai'er No.2'were corn flour and soybean flour,with appropriate addition amounts of 3-5 g·L-1 for both.The optimal formula for shake-flask spawn consisted of 5 g·L-1 corn flour,5 g·L-1 soybean flour,1.5 g·L-1 KH2 PO4,and 0.5 g·L-1 MgSO4.The optimal culture conditions were set at 28℃ for 9 days with a shaking speed of 170 r·min-1,and a liquid loading volume of 150 mL in a 250 mL erlenmeyer flask.For 1 000 mL shake flasks,the suitable inoculum size was 1-3 mycelium blocks of 1 cm2 each,and the optimal harvesting period was 6-7 days.'Tai'er No.2'was adaptable to multi-substrate cultivation modes in northern China.