不同保藏方法对灵芝全生产过程的影响OA

Ten Ganoderma lucidum strains with significant differences in genetic,agronomic,and bioactive characteristics were used as test materials to investigate the effects of low-temperature slant preservation and liquid nitrogen cryopreservation on genetic stability,agronomic traits,and bioactive indicators throughout the entire production cycle of Ganoderma lucidum,in order to provide a reference for efficient preservation and utilization of Ganoderma lucidum germplasm resources.The results showed that two preservation methods achieved a 100% revival rate of the strains,with no significant differences observed in core indicators such as mycelial dry mass,dehydrogenase activity,polysaccharide content,as well as fruiting body yield and quality.Strains preserved by low-temperature slant method exhibited extremely short revival time(all within one day),and significantly outperformed those preserved by liquid nitrogen in mycelial growth rate,medium decolorization rate,triterpenoid content,and activities of ligninase and acid protease at the mother culture stage(i.e.,the secondary culture stage).In contrast,liquid nitrogen cryopreservation demonstrated morphological advantages in fruiting bodies,with cap length significantly greater than that of slant-preserved strains.ISSR analysis revealed identical amplification bands under the same primers for both preservation methods,with no variation detected.Microscopic observation of mycelial morphology confirmed the presence of clamp connections in both groups,and no mononuclear phenomenon occurred.In conclusion,both preservation methods effectively maintain the genetic stability of Ganoderma lucidum germplasm.Low-temperature slant preservation offers advantages in revival efficiency and early growth vigor,contributing to the preservation of initial mycelial vitality and metabolic activity,making it suitable for production processes requiring rapid initiation and high early growth efficiency.Liquid nitrogen cryopreservation provides unique benefits in long-term genetic stability and potential optimization of fruiting body morphology.